PDGF-AA promotes cell-to-cell communication in osteocytes through PI3K/Akt signaling pathway  

作　　者：Yang Liu Mengmeng Duan Daimo Guo Shiyi Kan Li Zhang Munire Aili Demao Zhang Wei Du Jing Xie 

机构地区：[1]State Key Laboratory of Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610064,China [2]National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610064,China [3]Department of Cariology and Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610064,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第12期1640-1649,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.81600840 and 81771047 to J.X.and No.81900965 to W.D.).

摘　　要：Osteocytes are the main sensitive cells in bone remodeling due to their potent functional cell processes from the mineralized bone matrix to the bone surface&the bone marrow.Neighboring osteocytes communicate with each other by these cell processes to achieve molecular exchange through gap junction channels.Platelet-derived growth factor-AA(PDGF-AA)has been reported to enhance bone tissue remodeling by promoting cell proliferation,migration,&autocrine secretion in osteoid cell linage.However,the effect of PDGF-AA on intercellular communication between osteocytes is still unclear.In the present study,we elucidated that PDGF-AA could enhance the formation of dendritic processes of osteocytes&the gap junctional intercellular communication by promoting the expression of connexin43(Cx43).This modulation process was mainly dependent on the activation of phosphorylation of Akt protein by phosphatidylinositol 3-kinase(PI3K)/Akt(also known as protein kinase B,PKB)signaling.Inhibition of PI3K/Akt signaling decreased the Cx43 expression induced by PDGF-AA.These results establish a bridge between PDGF-AA&cell–cell communication in osteocytes,which could help us understand the molecular exchange between bone cells and fracture healing.

关 键 词：PDGF-AA cell communication CONNEXIN43 gap junction OSTEOCYTE 

分 类 号：R68[医药卫生—骨科学]