不同产地稻花香水稻代谢产物差异分析  

Analysis on the Difference of Daohuaxiang Rice Metabolites in Different Producing Areas

作　　者：冯玉超[1] 杨宏志[1,2] 安宇 张舒[1] 盛亚男 王长远 FENG Yuchao;YANG Hongzhi;AN Yu;ZHANG Shu;SHENG Yanan;WANG Changyuan(College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Chinese National Engineering Research Center,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《食品工业科技》2022年第1期10-20,共11页Science and Technology of Food Industry

基　　金：国家重点研发计划项目(2018YFE0206300);黑龙江省自然科学基金研究团队项目(TD2020C003);黑龙江省自然基金项目(LH2019C054);黑龙江八一农垦大学研究生创新项目(YJSCX2021-Z04)。

摘　　要：基于气相色谱与质谱联用(GC-MS)的代谢组学技术,对黑龙江省宁安市和五常市两个产地的水稻代谢产物与代谢差异进行研究。使用SIMCA软件和R软件对数据进行多元统计分析。结果表明,产地对稻花香水稻代谢物的数量、含量、种类及种类占比均具有影响,两产地水稻样本共定性到127个代谢物,筛选出22个差异代谢物,且发现产地对脂肪酸及其衍生物含量的影响最明显。代谢途径分析结果表明,不同产地间水稻代谢差异机制主要在于脂肪酸类物质的代谢,亚油酸、棕榈酸和富马酸是三种关键代谢物。本研究从代谢产物组成方面对不同产地的稻花香水稻代谢差异进行分析,可为水稻产地区分及农产品溯源提供理论支撑。Based on the metabolomic technique of GC-MS, the metabolites and metabolic differential mechanism of rice from Ning’an and Wuchang city were studied. SIMCA and R software were used for multivariate statistical analysis of the data. The results showed that the place of production had an influence on the quantity, content, type and proportion of the metabolites of Daohuaxiang rice. A total of 127 metabolites were identified in rice samples from the two regions. A total of 22 different metabolites were screened out through the analysis of the differences between the samples from the two regions. And it was found that the origin had the most significant influence on the contents of fatty acids and their derivatives. The results of metabolic pathway analysis showed that the main mechanism of rice metabolism difference in different producing areas was the metabolism of fatty acids. Linoleic acid, palmitic acid and fumaric acid were the three key metabolites. And the difference of metabolites type and content was related to rice quality. The research analyzes the different mechanisms of rice metabolism in different producing areas from the aspect of metabolite composition, which can provide theoretical support for the regional differentiation of rice production and the traceability of agricultural products.

关 键 词：产地 代谢组学 水稻 气相色谱与质谱联用(GC-MS) 差异机制 

分 类 号：TS21[轻工技术与工程—粮食、油脂及植物蛋白工程]