基于HPLC-MS/MS对枇杷叶水提组分分析及萜类物质的结构鉴定  

HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids

作　　者：李佳美 徐伟[1] 张雪 邵百琪 谢红瑶 吴凡 马智宇 陈华 LI Jiamei;XU Wei;ZHANG Xue;SHAO Baiqi;XIE Hongyao;WU Fan;MA Zhiyu;CHEN Hua(School of Food Engineering,Harbin University of Commerce,Harbin 150076,China;Nantong Tianlai Village Agricultural Science and Technology Development Co.,Ltd.,Haimen 226100,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076 [2]南通市天籁村农业科技发展有限公司,江苏海门226100

出　　处：《食品工业科技》2022年第1期295-303,共9页Science and Technology of Food Industry

基　　金：企业横向课题(201703210300804)。

摘　　要：利用高效液相色谱-质谱(HPLC-MS/MS)技术,对枇杷叶水提组分进行分析,并且对萜类物质进行解析。采用C18色谱柱(2.1 mm×100 mm,1.8μm),以水+0.1%甲酸溶液为流动相梯度洗脱,使用ESI离子源,在正、负离子模式下采集质谱数据,共检测到120个化合物;包括18种萜类化合物,总相对含量1452.693μg/mL;24种黄酮类化合物,总相对含量10378.081μg/mL;18种酚类化合物、总相对含量36967.955μg/mL;5种香豆素类化合物,总相对含量214.778μg/mL;进一步鉴定出18种萜类化合物的结构,主要包括乌苏烷型6种、齐墩果烷型7种、3种倍半萜、1种二萜类物质和1种特殊的环烯醚萜苷类物质;其中栎瘿酸、银杏内酯B、Prespatane、山栀苷甲酯、Icariside B5、皂皮酸、积雪草酸、18β-甘草次酸在以往的枇杷叶活性物质中未见报道。本文为枇杷叶水提成分的研究提供参考。Using high performance liquid chromatography-mass spectrometry(HPLC-MS/MS) technology, the components in the water extract of loquat leaves were analyzed, and the terpenoids were analyzed. Using a C18 column(2.1 mm×100 mm, 1.8 μm), using water +0.1% formic acid solution as mobile phase gradient elution, using ESI ion source, collecting mass spectrometry data in positive and negative ion modes, a total of 120 compounds were detected;Including 18 kinds of terpenoids with a total relative content of 1452.693 μg/mL;24 kinds of flavonoids with a total relative content of 10378.081μg/mL;18 kinds of phenolic compounds with a total relative content of 36967.955 μg/mL;5 kinds of coumarins compounds with a total relative content of 214.778 μg/mL;The structures of 18 terpenoids were further identified, including 6 ursane type, 7 oleanane type, 3 sesquiterpenes, and 1 diterpenoid. And a special iridoid glycosides;Roburic acid, ginkgolide B,prespatane, shanzhiside methyl ester, icariside B5, quillaic acid, asiatic acid, 18β-glycyrrhetinic acid were no report in the previous active substances of loquat leaves. This article provides a reference for the research on the water-extracted components of loquat leaves.

关 键 词：枇杷叶 水提组分 HPLC-MS/MS 萜类物质 结构鉴定 

分 类 号：TS202.1[轻工技术与工程—食品科学]