Icaritin Attenuates Lipid Accumulation by Increasing Energy Expenditure and Autophagy Regulated by Phosphorylating AMPK  

作　　者：Yue Wu Ying Yang Fang Li Jie Zou Yu-Hao Wang Meng-Xia Xu Yong-Lun Wang Rui-Xi Li Yu-Ting Sun Shun Lu Yuan-Yuan Zhang Xiao-Dong Sun 

机构地区：[1]West China School of Basic Medical Sciences&Forensic Medicine,Sichuan University,Chengdu,Sichuan,China [2]Department of Medical Oncology,Sichuan Cancer Hospital&Institute,School of Medicine,University of Electronic Science and Technology of China,Chengdu,Sichuan,China [3]Department of Radiation Oncology,Sichuan Cancer Hospital&Institute,Sichuan Cancer Center,School of Medicine,University of Electronic Science and Technology of China,Chengdu,Sichuan,China [4]Department of Radiological Protection,Radiation Oncology Key Laboratory of Sichuan Province,Chengdu,Sichuan,China [5]Department of Gastroenterology,The Second Affiliated Hospital of Chengdu Medical College,China National Nuclear Corporation 416 Hospital,Chengdu,Sichuan,China [6]State Key Laboratory of Medicinal Chemical Biology,Nankai University,Tianjin,China

出　　处：《Journal of Clinical and Translational Hepatology》2021年第3期373-383,共11页临床与转化肝病杂志（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Grant No.81770580);open funding from the Sichuan Provincial Key Laboratory of Radiation Oncology(Grant No.2020FSZLX-02).

摘　　要：Background and Aims:Lipid accumulation is the major characteristic of non-alcoholic fatty liver disease,the prevalence of which continues to rise.We aimed to investigate the effects and mechanisms of icaritin on lipid accumulation.Methods:Cells were treated with icaritin at 0.7,2.2,6.7,or 20μM for 24 h.The effects on lipid accumulation in L02 and Huh-7 cells were detected by Bodipy and oil red O staining,respectively.Mitochondria biogenesis of L02 cells was detected by MitoTracker Orange staining.Glucose uptake and adenosine triphosphate content of 3T3-L1 adipocytes and C2C12 myotubes were detected.The expression levels of proteins in the adenosine 5′-monophosphate-activated protein kinase(AMPK)signaling pathway,biomarkers of autophagy,and mitochondria biogenesis were measured by western blotting.LC3 puncta were detected by immunofluorescence.Results:Icaritin significantly attenuated lipid accumulation in L02 and Huh-7 cells and boosted the mitochondria biogenesis of L02 cells.Icaritin enhanced glucose uptake,decreased adenosine triphosphate content,and activated the AMPK signaling pathway in 3T3-L1 adipocytes and C2C12 myotubes.Icaritin boosted autophagy and also enhanced the initiation of autophagic flux in 3T3-L1 preadipocytes and C2C12 myoblasts.However,icaritin decreased autophagy and promoted mitochondria biogenesis in 3T3-L1 adipocytes and C2C12 myotubes.Conclusions:Icaritin attenuates lipid accumulation by increasing energy expenditure and regulating autophagy by activating the AMPK pathway.

关 键 词：ICARITIN Lipid accumulation NAFLD AMPK AUTOPHAGY 

分 类 号：R58[医药卫生—内分泌]