紫色红曲霉Mp-21次级代谢产物抗氧化及抑制α-葡萄糖苷酶活性的研究  

Antioxidation and inhibition ofα-glucosidase activity of secondary metabolites of Monascus purpureus Mp-21

作　　者：丁允章 孔黎春[1] 张奕嘉 蒋冬花[1] DING Yunzhang;KONG Lichun;ZHANG Yijia;JIANG Donghua(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,Zhejiang,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《微生物学报》2022年第1期103-118,共16页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31570013)。

摘　　要：【目的】紫色红曲霉(Monascus purpureus)Mp-21次级代谢产物的分离纯化与生物活性研究。【方法】综合运用硅胶柱、反相C_(18)柱和Sephadex LH-20凝胶柱等柱色谱分离技术对紫色红曲霉Mp-21的次级代谢产物进行分离纯化,运用波谱学技术(核磁共振和高分辨质谱技术)鉴定化合物的结构。对鉴定的化合物进行抗氧化与降血糖相关酶抑制活性的测定。【结果】从紫色红曲霉Mp-21次级代谢产物中鉴定出10个活性化合物,分别为FMOC-ALLO-THR(TBU)-OH(1)、quercetin(2)、hesperetin(3)、monascin(4)、monasphilone A(5)、oleanolic acid(6)、β-sitosterol(7)、ergosterol(8)、indole-3-carboxylic acid(9)、chlorogenic acid(10)。其中化合物1为新天然产物,补充了该化合物在自然界中的信息和有机波谱信息,化合物2、6、9、10首次在红曲菌科中发现。在体外清除O2–、DPPH和OH–自由基的抗氧化活性试验中,化合物2(IC_(50)分别为5.07、4.84和27.39μg/mL)表现出较强的抗氧化能力。通过电子顺磁共振技术验证了化合物2对DPPH和OH–自由基具有较强的清除能力。化合物6对α-葡萄糖苷酶具有较强的抑制作用,IC_(50)为22.87μg/mL。【结论】紫色红曲霉Mp-21是极具开发性能的优质种质资源,具有开发成抗氧化、降血糖等功能性食品的潜力。[Objective]To study the separation,purification and biological activity of secondary metabolites from Monascus purpureus Mp-21.[Methods]The secondary metabolites of Monascus purpureus Mp-21 were isolated and purified using silica gel column,RPC18 column and Sephadex LH-20 gel column and other column chromatographic separation techniques,and the structure of the compound was identified by spectroscopy technique(NMR and HR-ESI-MS).The anti-oxidation and hypoglycemic-related enzyme inhibitory activities of the identified compounds were determined.[Results]Ten active compounds were identified from the secondary metabolites of Monascus purpureus Mp-21,namely FMOC-ALLO-THR(TBU)-OH(1),quercetin(2),hesperetin(3),monascin(4),monasphilone A(5),oleanolic acid(6),β-sitosterol(7),ergosterol(8)、indole-3-carboxylic acid(9)and chlorogenic acid(10).Among them,compound 1 is a new natural product,which complements the information of the compound in nature and the information of the organic spectrum.Compounds 2,6,9 and 10 are discovered from the Monascus for the first time.In the in vitro antioxidant activity tests of O2–,DPPH and OH–free radicals,compound 2(IC50 of 5.07,4.84 and 27.39μg/mL,respectively)showed strong antioxidant capacity.Electron paramagnetic resonance(EPR)showed that compound 2 had strong scavenging ability on DPPH and OH–.Compound 6 had strong inhibitory activity onα-glucosidase with IC50 of 22.87μg/mL.[Conclusion]Monascus purpureus Mp-21 is a high-quality germplasm resource with high development performance,and has the potential to be developed into functional food such as antioxidant and hypoglycemic.

关 键 词：紫色红曲霉 新天然产物 抗氧化 降血糖 

分 类 号：TS201.2[轻工技术与工程—食品科学]