溃疡性结肠炎患者活动期肠道菌群及粪便炎症标志物检测及其临床意义  

Detection of intestinal flora and fecal inflammation markers in patients with ulcerative colitis during active period and its clinical significance

作　　者：陈茜茜 朱海勇 CHEN Xixi;ZHU Haiyong(Endoscopy Center,Taizhou Central Hospital(Taizhou University Hospital),Taizhou 318000,China;Intensive Care Unit,Medical Community of Linhai First People's Hospital,Taizhou 317000,China)

机构地区：[1]台州市中心医院(台州学院附属医院)内镜中心,浙江台州318000 [2]临海市第一人民医院ICU,浙江台州317000

出　　处：《中国现代医生》2021年第34期16-19,共4页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2019KY242)。

摘　　要：目的观察溃疡性结肠炎患者活动期肠道菌群及粪便炎症标志物含量及其临床意义。方法回顾性分析2018年3月至2021年1月在我院治疗的60例溃疡性结肠炎患者的临床资料,按照病情状况分为活动期组(n=39)与缓解期组(n=21),同时选择在我院体检的50名健康者为对照组。比较三组研究对象的肠道需氧菌群(包括酵母菌、肠球菌、葡萄球菌、肠杆菌)、厌氧菌群(包括双歧杆菌、拟杆菌、乳杆菌、真杆菌、消化球菌)以及粪便炎症标志物乳铁蛋白(LF)、钙卫蛋白(FC)、髓过氧化酶(MPO)、基质金属蛋白酶-9(MMP-9)的水平。结果活动期组酵母菌、肠球菌、葡萄球菌、肠杆菌数量明显高于缓解期组与对照组,缓解期组酵母菌、肠球菌、肠杆菌数量明显高于对照组(P<0.05);活动期组双歧杆菌、乳杆菌、真杆菌明显低于缓解期组与对照组,拟杆菌、消化球菌明显高于缓解期组与对照组,缓解期组双歧杆菌、乳杆菌、真杆菌明显低于对照组,拟杆菌、消化球菌明显高于对照组(P<0.05);活动期组LF、FC、MPO、MMP-9水平明显高于缓解期组与对照组,缓解期组LF、FC、MPO、MMP-9水平明显高于对照组(P<0.05)。结论溃疡性结肠炎患者肠道菌群失调,粪便中炎症标志物水平明显较高,能够通过肠道菌群变化及粪便内LF、FC、MPO、MMP-9水平评估疾病的活动性。Objective To observe the levels of intestinal flora and fecal inflammation markers in patients with ulcerative colitis and its clinical significance.Methods The clinical data of 60 patients with ulcerative colitis admitted to our hospital from March 2018 to January 2021 were retrospectively analyzed.According to their condition,the patients were divided into the active period group(n=39)and the remission period group(n=21).At the same time,50 healthy people who had a physical examination in our hospital were selected as the control group.The intestinal aerobic flora(including yeast,enterococcus,staphylococcus,and enterobacteriaceae),the anaerobic flora(including bifidobacterium,bacteroides,lactobacillus,eubacterium,peptococcus),and the fecal inflammation markers lactoferrin(LF),calprotectin(FC),myeloperoxidase(MPO),matrix metalloproteinase-9(MMP-9)between the three groups were compared and analyzed.Results The numbers of enterococcus,staphylococci,and enterobacteriaceae in the active stage group were significantly higher than those in the remission stage group and the control group.The numbers of yeast,enterococcus,and enterobacteriaceae in the remission stage group were significantly higher than those in the control group.The differences were statistically significant(P<0.05).The bifidobacterium,lactobacillus,eubacteria in the active period group were significantly lower than those in the remission stage group and the control group.The bacteroides and peptococcus in the active period group were significantly higher than those in the remission stage group and the control group.The bifidobacterium,lactobacillus,and eubacteria in the remission stage group were significantly lower than those in the control group.The bacteroides and peptococcus in the remission stage group were significantly higher than those in the control group.The differences were statistically significant(P<0.05).The levels of LF,FC,MPO,and MMP-9 in the active stage group were significantly higher than those in the remission group and the control

关 键 词：溃疡性结肠炎 活动期 需氧菌群 厌氧菌群 乳铁蛋白 钙卫蛋白 髓过氧化酶 基质金属蛋白酶-9 

分 类 号：R574.62[医药卫生—消化系统]