基于核酸适配体的胶体金层析试纸条对镉离子的定量检测  

Quantitative Determination of Cadmium Ion Based on Gold Nanoparticle-labeled Aptamer Lateral Flow Test Strip

作　　者：涂东堃 郑丽婷 郑晓亮 王艺伟[1] 曾绍校[1] 徐晖[1] Tu Dongkun;Zheng Liting;Zheng Xiaoliang;Wang Yiwei;Zeng Shaoxiao;Xu Hui(College of Food Science,Fujian Agriculture and Forestry University,Fujian,350002)

机构地区：[1]福建农林大学食品科学学院,福建350002

出　　处：《当代化工研究》2021年第24期30-32,共3页Modern Chemical Research

基　　金：2019年福建农林大学科技创新专项基金项目:“碳基荧光探针的构建及其对重金属检测的研究”(项目编号:CXZX2019098S);2019年福建省科技厅自然科学基金青年创新项目“基于靶标诱导核酸G-四链体构象变化的镉离子传感器的研究”(项目编号:2019J05046)。

摘　　要：建立了一种竞争性胶体金标记核酸试纸条用于Cd^(2+)的快速检测。方法制备胶体金标记核酸复合物,研究核酸适配体和检测探针对试纸条分析性能的影响。试纸条在Cd^(2+)浓度为10~100ng/mL时呈现出良好的线性关系,检测限为3.79ng/mL。以核酸适配体为分子识别元件建立了一种胶体金标记核酸适配体试纸条检测方法,该试纸条对Cd^(2+)具有较好的特异性和稳定性,可用于镉离子的快速检测。A novel and efficient method for detecting Cadmium ion was developed based on aptamer lateral flow test strip,using gold nanoparticles(GNP)-labeled DNA probe.The GNP labeled aptamer complexes were prepared,and the analytical performance of test strip were optimized.The optimal preparation conditions of GNP labeled aptamer complexes were obtained.The test strip showed a good linear relationship with the Cd^(2+) concentration of 10-100 ng/mL,and the detection limit is 3.79 ng/mL.A test strip detection method was established using aptamer as molecular recognition elements.The test strip has good specificity and stability for Cd^(2+),and can be used for rapid detection of cadmium ions.

关 键 词：核酸适配体 镉离子 快速检测 试纸条 

分 类 号：TS207.3[轻工技术与工程—食品科学]