顶空固相微萃取结合气质联用方法快速测定地椒挥发性成分  

Rapid analysis of the volatile components of Mongolian thyme herb′s essential oils using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry

作　　者：李启东 赵金 孔娜 黄璐瑶 潘少斌[1] LI Qi-dong;ZHAO Jin;KONG Na;HUANG Lu-yao;PAN Shao-bin(School of Pharmaceutical Sciences,Shandong Provincial Collaborative Innovation Center for Quality Control and Construction of the Whole Industrial Chain of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Bluetown Analysis and Test Co.,Ltd.,Jinan 250102,China)

机构地区：[1]山东中医药大学药学院山东省高校中药质量控制与全产业链建设协同创新中心,山东济南250355 [2]山东蓝城分析测试有限公司,山东济南250102

出　　处：《山东科学》2022年第1期1-5,共5页Shandong Science

基　　金：山东省高校中药质量控制与全产业链建设协同创新中心资助课题(CYLXTCX2020-11)。

摘　　要：采用顶空固相微萃取法(headspace solid-phase microextraction,HS-SPME)结合气质联用技术(gas chromatography-mass spectrometry,GC-MS)提取、分析地椒中的挥发性化学成分,通过面积归一化法确定各成分的相对含量。结果检出挥发性成分56种,其中以酚醇类、萜类、烯类、酯类、醛酮类化合物为主,主要成分有芳樟醇(27.8%)、龙脑(9.12%)、樟脑萜(8.12%)、柠檬烯(7.81%)等。本实验首次对山东地区地椒挥发性成分进行HS-SPME结合GC-MS研究,发现其化学成分丰富,具有较高的药用价值。The headspace solid-phase microextraction(HS-SPME)method combined with gas chromatography-mass spectrometry(GC-MS)was used to extract and analyze the volatile components of Mongolian thyme herb,the peak areas were normalized to obtain the relative content of each component.A total of 56 volatile components were detected,of which,phenolic alcohols,terpenes,alkenes,esters,aldehydes,and ketones were the primary chemical compounds.linalool(27.8%),borneol(9.12%),camphor terpene(8.12%),limonene(7.81%),etc.were found to be the main ingredients.This is the first study wherein HS-SPME was performed in combination with GC-MS to analyze the volatile components of Mongolian thyme herb found in the Shandong area.The chemical components were found to be rich and have a high medicinal value.

关 键 词：地椒 顶空固相微萃取 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]