Celastrol promotes chondrocyte autophagy by regulating mTOR expression  

作　　者：Siming Dai Jiankun Fan Yue Zhang Zhenyong Hao Huiming Yu Zhiyi Zhang 

机构地区：[1]Department of Rheumatology and Immunology,The First Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang 150001,China [2]The Laboratory of Medical Genetics,Harbin Medical University,Harbin,Heilongjiang 150001,China [3]Shenzhen Futian Hospital for Rheumatic Diseases,Shenzhen,Guangdong 518000,China [4]Department of Orthopedic Surgery of Harbin Fifth Hospital,Harbin,Heilongjiang 150001,China

出　　处：《Chinese Medical Journal》2022年第1期92-94,共3页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(No.81771749 and No.81771748)。

摘　　要：Osteoarthritis(OA)is a debilitating disease with limited treatment options.[1]Autophagy serves as an important protective mechanism against the deterioration of cartilage and is negatively regulated by the mammalian target of rapamycin(mTOR).[2]In previous studies,celastrol could promote autophagy in various cell types and had the promising therapeutic potential for OA.[3]However,the effect and underlying mechanisms of celastrol on autophagy in OA remain unclear.Our preliminary study using transcriptome sequencing and a network pharmacology analysis identified mTOR as a potential direct target of celastrol for protection against OA.[4]In this study,we aim to evaluate the effects of celastrol on mTOR activity in primary human osteoarthritic chondrocytes and to determine the mechanism by which it affects autophagy and endoplasmic reticulum(ER)stress.

关 键 词：protective MTOR OSTEOARTHRITIS 

分 类 号：R684.3[医药卫生—骨科学]