淡紫灰链霉菌X33发酵提取物对采后柑橘绿霉病菌的抑制作用  

Inhibitory Effect of Fermentation Extract of Streptomyces lavendulae Strain X33 on Postharvest Citrus Green Mold

作　　者：林书华 汪林兰 罗攀 张斌 吴晓玉[1] Lin Shuhua;Wang Linlan;Luo Pan;Zhang Bin;Wu Xiaoyu(College of Bioscience and Bioengineering,Jiangxi Agriculture University Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Nanchang 330045)

机构地区：[1]江西农业大学生物科学与工程学院江西农业微生物资源开发与利用工程实验室江西省果蔬采后处理关键技术及质量安全协同创新中心,南昌330045

出　　处：《中国食品学报》2022年第1期206-214,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：江西省重点研发计划重点项目(20171ACF60006);农业部功能食品重点实验室开放基金项目(201606)。

摘　　要：为了揭示淡紫灰链霉菌X33发酵提取物(SLFE)对柑橘采后绿霉病菌——指状青霉Pd165的抑制作用,以SLFE处理的菌株Pd165为研究对象,测定病原菌菌株Pd165细胞完整性及相关细胞内容物的变化。采用扫描电镜、拉曼光谱、荧光光谱和荧光定量PCR,探究SLFE处理对菌丝形态、细胞应答和DNA的影响。结果表明:1.2 mg/mL SLFE处理导致菌株Pd165菌丝干瘪、扭曲折叠,分生孢子梗与分生孢子畸形,且产孢抑制率高达94.86%。SLFE诱导菌丝细胞完整性受损严重,导致胞外上清液中代表脂肪酸、核酸和蛋白质的特征峰增强(1 129,1 330,1 565 cm-1),胞内糖类、可溶性蛋白和丙酮酸含量下降。SLFE处理破坏了菌株Pd165基因组DNA构象和结构,上调了与细胞完整性、产孢、代谢和氧化应激相关的基因表达水平(cej60377.1、oko99898.1、crl18986.1和kzn90160.1)。结论:SLFE处理导致菌株Pd165细胞完整性受损,扰乱细胞内原有的稳态,并严重破坏DNA构象及菌丝体形态。In order to reveal the inhibitory effect of the extract of fermentation broth from Streptomyces lavendulae X33(hereafter referred to as SLFE) on Penicillium digitatum(P.digitatum),a postharvest pathogen of citrus,the cellular integrity and related cell contents of P.digitatum treated with SLFE were determined.Scanning electron microscope,Raman spectrum,fluorescence probe,fluorescence spectrum and fluorescence quantitative PCR were used to observe the effects of SLFE treatment on mycelium morphology,cell response and DNA.The results showed that the hyphae of the strain Pd165 appeared withered,twisted and folded,and the conidiophores and conidia were abnormal by the treatment of 1.2 mg/mL SLFE,and moreover,the sporulation inhibition rate was as high as 94.86%.At the same time,the cell integrity of the strain was seriously damaged by SLFE,which acted as triggers for an increase in the characteristic peaks of fatty acids,nucleic acids and proteins in the extracellular supernatant(1 129,1 330,1 565 cm-1),and the massive exudation of intracellular sugars,nucleic acids and soluble proteins,and a decrease of the pyruvate content.In addition,SLFE treatment destroyed the conformation and structure of P.digitatum genomic DNA and upregulated the expression levels of genes related to cell integrity,sporulation,metabolism and oxidative stress(cej60377.1,oko99898.1,crl18986.1,kzn90160.1).The results suggested that SLFE treatment damaged the integrity of P.digitatum cells,disturbed the original homeostasis of cells,and seriously destroyed the conformation of DNA and the morphology of mycelium.

关 键 词：淡紫灰链霉菌X33发酵提取物 指状青霉Pd165 抑制作用 细胞完整性 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]