绿茶、白茶水提物对肥胖小鼠血糖、血脂的干预作用及肠道微生物的影响  

Effects of green tea and white tea water extracts on blood glucose and blood lipids in obese mice intervention and the influence of intestinal microbes

作　　者：汪长钢[1] 于佳弘 潘妍[1] WANG Changgang;YU Jiahong;PAN Yan(Beijing Agricultural Vocational College,Beijing 102442;Weihai Marine Vocational College,Weihai 264300)

机构地区：[1]北京农业职业学院,北京102442 [2]威海海洋职业学院,威海264300

出　　处：《中国食品添加剂》2022年第1期113-122,共10页China Food Additives

基　　金：北京市教委课题“红茶、绿茶提取物对肠道微生物影响的研究”(编号:KM201612448004);特高校建设专项特色高水平院校建设项目-打造技术创新平台-食品营养与安全应用技术协同创新中心(PXM2021_157102_000005)。

摘　　要：实验比较不同剂量白茶水提物和绿茶水提物对肥胖小鼠降糖、降脂的效果及对小鼠肠道菌群的影响。随机将48只小鼠分为正常组(CK)6只小鼠、造模组(HFD)6只和造模干预组36只小鼠。造模组分别采用2.5%、5%、10%白茶水提物和绿茶水提物处理。实验结束测定小鼠血糖、血脂,并通过高通量测序技术研究小鼠肠道微生物菌属相对丰度变化。结果显示:绿茶和白茶水提物只有剂量达到5%才能够降低小鼠体重和脂肪总重,且绿茶水提物的减脂效果更好。两者都能降低TG、TC、LDL-C含量,5%的绿茶和白茶显著降低LDL-C含量,5%的绿茶和白茶水提物降低TG的效果较好,只有10%剂量的绿茶水提物能显著降低TC效果。5%的绿茶水提物和白茶水提物有较好的降血糖效果。绿茶水提物肠道微生物实验结果分析显示Lachnoclostridium、Rikenellaceae_RC9_gut_group菌属丰度在2.5%~5%区间是逐步上升的,白茶水提物有剂量效应的菌属为Lachnoclostridium、Tyzzerella、Lachnospiraceae_UCG_006。The experiment compares the effects of different doses of white tea water extract and green tea water extract on reducing blood sugar and lipids in obese mice,and the effect on the intestinal flora of mice.The 48 mice were randomly divided into 6 mice in the normal group(CK)and 42 mice in the model group.The manufacturing modules are treated with 2.5%,5%,10%white tea water extract and green tea water extract respectively.At the end of the experiment,the blood glucose and blood lipids of the mice were measured,and the relative abundance of the intestinal microbes of the mice were studied by high-throughput sequencing technology.The results showed that the water extract of green tea and white tea can reduce the body weight and total fat of mice only when the dosage reaches 5%,and the water extract of green tea has a better fat reduction effect.Both can reduce the content of TG,TC,LDL-C,5%green tea and white tea significantly reduce the content of LDL-C,5%green tea and white tea water extracts have a better effect on reducing TG,only 10%green tea water The extract can significantly reduce the TC effect.5%green tea water extract and white tea water extract have better hypoglycemic effect.The analysis of the intestinal microbial experiment results of the water extract of green tea showed that the abundance of Lachnoclostridium and Rikenellaceae_RC9_gut_group gradually increased in the range of 2.5%~5%.The bacteria of the water extract of white tea were Lachnoclostridium,Tyzzerella,Lachnospiraceae_UCG_006.

关 键 词：白茶水提物 绿茶水提物 血糖 血脂 肠道微生物 

分 类 号：TS202.1[轻工技术与工程—食品科学] R151.1[轻工技术与工程—食品科学与工程]