表没食子儿茶素没食子酸酯对短肠综合征大鼠肠道代偿和肠道屏障的影响  

Effect of epigallocatechin gallate on intestinal adaptation and intestinal barrier in a rat model of short bowel syndrome

作　　者：周正明 李可 汪云风 胡雯[1,2] ZHOU Zheng-ming;LI Ke;WANG Yun-feng;HU Wen(West China School of Public Health/West China Fourth Hospital,Sichuan University,Chengdu,Sichuan 610041,China;不详)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,四川成都610041 [2]四川大学华西医院临床营养科,四川成都610041

出　　处：《现代预防医学》2022年第2期333-338,共6页Modern Preventive Medicine

基　　金：四川省科技厅科技计划项目(2020YJ0040)。

摘　　要：目的观察研究表没食子儿茶素没食子酸酯(EGCG)对空肠切除后的短肠综合征(SBS)模型大鼠剩余肠道代偿功能和肠道屏障的影响。方法 32只SPF级健康雄性SD大鼠,平均随机分为4组,每组8只,分别为假手术组、手术对照组、低剂量EGCG手术组和高剂量EGCG手术组。假手术组进行空肠横断吻合,其余手术组均切除中段小肠,形成SBS模型。假手术组和手术对照组术后2~14天灌胃生理盐水,低剂量和高剂量EGCG手术组分别灌胃等体积50 mg/kg和100 mg/kg EGCG,术后第15 d处死大鼠。测定各组大鼠术后各组大鼠体重变化;血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)水平;ELISA法检测回肠组织肿瘤坏死因子(TNF-α)和白细胞介素6(IL-6)的水平;分析小肠病理切片的绒毛长度和隐窝深度评估肠道代偿功能;采用免疫荧光法检测紧密连接蛋白claudin-1和occludin的分布和平均荧光强度(MFI);RT-q PCR方法检测大鼠回肠组织TLR4、My D88和NF-κB p65 m RNA相对表达量。结果与手术对照组比较,各剂量EGCG手术组大鼠体重均增加(P<0.05);血清SOD、CAT的活性和MDA含量增加(P<0.05);小肠绒毛高度和隐窝深度增加(P<0.05);回肠组织TNF-α和IL-6的水平减少(P<0.05);回肠黏膜紧密连接蛋白claudin-1和occludin的MFI增加(P<0.05),回肠组织TLR4、My D88和NF-κB p65 m RNA相对表达量减少(P<0.05)。结论 EGCG可以通过抑制TLR4/NF-κB信号通路的活化,减轻SBS大鼠的氧化应激和组织炎症,促进小肠功能恢复和保护肠道屏障,加快术后的肠适应和肠康复。Objective To investigate the effect of epigallocatechin gallate(EGCG) on the residual intestinal compensatory function and intestinal barrier of the short bowel syndrome(SBS) rat after jejunectomy.Methods In total 32 SPF-grade healthy male SD rats were randomly divided into four groups,with eight rats in each group.The sham group underwent transected jejunum anastomosis,and the rest of group were resected in the middle of the small intestine to form SBS models.The sham group and the control group were given normal saline daily for 2 to 14 days after the operation,and the low-dose and high-dose EGCG groups were given the equal volume of 50 mg/kg and 100 mg/kg EGCG,respectively.The rats were sacrificed on 15 thday.The body weight changes of each group of rats after operation were measured.Serum SOD,CAT activity and MDA level were measured.Ileal tissue TNF-α and IL-6 levels were detected by ELISA.The intestinal adaptation function was evaluated by analyzing villus length and crypt depth.The distribution and MFI of claudin-1 and occludin were de-tected by immunofluorescence.RT-qPCR method was used to detect the relative expression of TLR4,MyD88 and NF-κB p65 m RNA in ileum tissue.Results Compared with the control group,the weight of rats in each dose of EGCG group increased(P<0.05);the activity of serum SOD,CAT and the level of MDA increased(P<0.05);the small intestine villus height and crypt depth increased(P<0.05);TNF-α and IL-6 levels in ileum tissue decreased(P<0.05);MFI of claudin-1 and occludin increased(P<0.05);the relative expression of TLR4,MyD88 and NF-κB p65 mRNA in ileal tissue was decreased(P<0.05).Conclusion EGCG can significantly reduce the oxidative stress and tissue inflammation of SBS,promote the recovery of small intestine function and protect the intestinal barrier by inhibiting the TLR4/NF-κB signaling pathway,and accelerate postoperative intestinal adaptation and intestinal rehabilitation.

关 键 词：表没食子儿茶素没食子酸酯 短肠综合征 肠道代偿 肠道屏障 TLR4/MyD88信号通路 

分 类 号：R151.2[医药卫生—营养与食品卫生学]