高效氯氰菊酯在甘蓝中的残留行为及膳食风险评估  

Residue Behavior and Dietary Risk Assessment of Beta-cypermethrin in Cabbage

作　　者：苏龙[1,2] 龚道新[1,2] 赵佳[1,2] 廖婵娟 SU Long;GONG Daoxin;ZHAO Jia;LIAO Chanjuan(College of Resources and Environment,Hunan Agricultural University,Changsha 410128,China;Institute of Agricultural Environmental Protection,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学资源环境学院,湖南长沙410128 [2]湖南农业大学农业环境保护研究所,湖南长沙410128

出　　处：《食品科学》2022年第1期150-155,共6页Food Science

基　　金：湖南省教育厅优秀青年项目(20B304);湖南省教育厅科学研究项目(18C0142)。

摘　　要：为了评价高效氯氰菊酯在甘蓝上的使用安全性,在陕西、福建等十二地进行了田间试验,研究高效氯氰菊酯在甘蓝中的残留及消解动态,并进行膳食风险评估。甘蓝样品采用乙腈提取,经抽滤、盐析、浓缩后,正己烷定容至5 mL,进行气相色谱检测。结果表明:在0.01~5.00 mg/L范围内,高效氯氰菊酯质量浓度与其响应峰面积呈良好的线性关系,决定系数为0.9999。在添加水平为0.05、0.5、5 mg/kg时,高效氯氰菊酯在甘蓝中的添加回收率范围在83%~106%之间,相对标准偏差为6%~9%,方法定量限为0.05 mg/kg。3%高效氯氰菊酯微囊悬浮剂在甘蓝上施药浓度为33.75 g/hm2,施药2次,施药间隔期7 d,末次施药后7 d时,甘蓝样品中高效氯氰菊酯的残留量为<0.05 mg/kg(低于定量限)~0.28 mg/kg,规范残留试验中值(standard test median residue,STMR)为0.13 mg/kg;末次施药后10 d时,甘蓝样品中高效氯氰菊酯的残留量为<0.05 mg/kg(低于定量限)~0.21 mg/kg,STMR为0.07 mg/kg,残留量均低于高效氯氰菊酯在甘蓝中的最大残留限量(maximum residue limit,MRL)(5 mg/kg)。高效氯氰菊酯在甘蓝中的残留消解动态规律符合一级动力学反应模型,山西、浙江、湖南和广西四地高效氯氰菊酯在甘蓝中的消解半衰期分别为3.8、2.0、2.7、3.7 d,相关系数在0.967~0.994之间。高效氯氰菊酯的国家估算每日摄入量(national estimated daily intake,NEDI)为0.575 mg,风险商为45.6%,风险评估结果表明高效氯氰菊酯膳食摄入风险处于可接受水平。In order to evaluate the safety of beta-cypermethrin applied on cabbage(Brassica oleracea L.),the residue and dissipation dynamics of beta-cypermethrin in cabbage were studied through filed experiments carried out in Shaanxi,Fujian and ten other provinces in China and the dietary intake risk of beta-cypermethrin was assessed.Samples were extracted with acetonitrile.The extract was suction filtrated,salted out,concentrated and then brought to a final volume of 5 mL with n-hexane before analysis by gas chromatography(GC).The results showed that good linearity was found between the concentration of beta-cypermethrin in the range of 0.01–5.00 mg/L and response peak area,with a determination coefficient of 0.9999.The recoveries of beta-cypermethrin at three spiked levels of 0.05,0.5 and 5 mg/kg ranged from 83%to 106%,with relative standard deviation(RSD)of 6%–9%.The limit of quantitation(LOQ)of the developed method was 0.05 mg/kg.In filed experiments,3%beta-cypermethrin microcapsule suspension was applied twice times on cabbage at a dose of 33.75 g/hm2 with an interval of seven days.At seven days after the last application,the residue of beta-cypermethrin in cabbage ranged between<0.05 mg/kg(less than LOQ)and 0.28 mg/kg,and the supervised trials median residue(STMR)was 0.13 mg/kg.At 10 days after the last application,the residue of beta-cypermethrin in cabbage ranged from<0.05 mg/kg(less than LOQ)and 0.21 mg/kg,and the STMR was 0.07 mg/kg.Both residue levels were lower than the maximum residue limit(MRL)value of 5 mg/kg.The dissipation process of beta-cypermethrin in cabbage followed a first-order kinetic model.The dissipation half-lives of beta-cypermethrin residues in cabbage from Shanxi,Zhejiang,Hunan and Guangxi were 3.8,2.0,2.7 and 3.7 days,respectively,and the correlation coefficients(r)ranged from 0.967 to 0.994.The national estimated daily intake(NEDI)of beta-cypermethrin was 0.575 mg and the risk quotient(RQ)was 45.6%.The results of risk assessment showed that the dietary intake risk of beta-cypermethrin

关 键 词：高效氯氰菊酯 甘蓝 气相色谱法 残留 膳食风险评估 

分 类 号：TS201.6[轻工技术与工程—食品科学]