小麦酯酶电化学生物传感器构建及其对敌敌畏的检测  

Construction of Wheat Esterase Electrochemical Biosensor and Its Application in Dichlorvos Detection

作　　者：刘丰 田运霞 吴远根 陶菡 LIU Feng;TIAN Yunxia;WU Yuangen;TAO Han(Provincial Key Laboratory of Fermentation Engineering and Biopharmacy,College of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,发酵工程与生物制药省重点实验室,贵州贵阳550025

出　　处：《食品科学》2022年第2期355-360,共6页Food Science

基　　金：贵州省科技计划项目(黔科合LH字[2017]7294号);国家自然科学基金地区科学基金项目(31760486);贵州省科技支撑计划项目(黔科合支撑[2019]2382号;黔科合支撑[2018]2795号);贵州大学2017年度学术新苗培养及创新探索专项(黔科合平台人才[2017]5788)。

摘　　要：以来源于植物的小麦酯酶(plant-esterase from wheat flour,wPLaE)为检测酶源,利用纳米金(gold nanoparticles,AuNPs)和多壁碳纳米管(multi-walled carbon nanotubes,MWNT)的协同电催化放大作用,构建一种可用于有机磷农药检测的新型电化学酶传感器NF/wPLaE-CS/AuNPs/MWNT/GCE。以敌敌畏为对象,采用方波伏安法实现对其简便、低成本的检测,检出限为4μg/L(R_(SN)=3)。将其应用于实际样品生菜和大米中敌敌畏的检测,回收率为95.4%~107.2%,表明所制备的植物酯酶生物传感器在实际应用中具有可行性。此外,该生物传感器也具有良好的稳定性和抗干扰能力。本研究可为植物酯酶在农药检测中的应用开发提供技术支撑。A new enzyme-based electrochemical sensor NF/wPlaE-CS/AuNPs/MWNT/GCE was constructed using wheat esterase by taking advantage of the synergistic electro-catalytic amplification effect of gold nanoparticles(AuNPs)and multi-walled carbon nanotubes(MWNT)on the electrochemical signal.The sensor provided a simple and low-cost approach for the detection of dichlorvos with square wave voltammetry,and the detection limit was 4μg/L(R_(SN)=3).The biosensor was used to measure dichlorvos spiked in lettuce and rice samples with recoveries ranging from 95.4%to 107.2%,indicating the biosensor was suitable for the detection of dichlorvos pesticides in actual samples.In addition,the biosensor exhibited good stability and inference resistance.This study can provide technical support for the application of plant esterase in pesticide detection.

关 键 词：敌敌畏 植物酯酶 小麦 多壁碳纳米管 纳米金 电化学生物传感器 

分 类 号：O657.1[理学—分析化学] TS201.6[理学—化学]