山东汉族人群中发现一例罕见的血小板特异性抗原HPA-10bw等位基因  

Identification of a rare platelet-specific antigen HPA-10bw allele among ethnic Han Chinese population in Shandong

作　　者：邵静茹 李文超[1] 潘英芳 乔文本 朱传福[1] 聂向民[1] 刘艳[1] Shao Jingru;Li Wenchao;Pun Yingfang;Qiao Wenben;Zhu Chuanfu;Nie Xiangmin;Liu Yan(Blood Center of Shandong Province,Jinan,Shandong 250014,China)

机构地区：[1]山东省血液中心,济南250014

出　　处：《中华医学遗传学杂志》2022年第2期231-233,共3页Chinese Journal of Medical Genetics

摘　　要：目的探讨山东汉族人群血小板抗原系统10(HPA-10w)的基因多态性, 补充本地区血小板供者库数据。方法应用PCR特异性引物分析法(PCR sequence specific primer)和直接测序方法对山东地区汉族血小板捐献者样本进行HPA-10等位基因分型。结果在1401例山东汉族机采血小板捐献者中, 发现一例罕见的HPA-10w(a+b+)杂合子携带者。HPA-10bw等位基因频率在山东汉族人群中约为0.035%。结论山东汉族人群中HPA-10bw低频抗原的检出, 对诊断和预防新生儿同种免疫血小板减少症(neonatal alloimmune thrombocytopenic purpura, NAIT)以及输血后紫癜(post-transfusion purpura, PTP)具有临床意义。Objective To study the polymorphism of human platelet antigen(HPA)system 10 among ethnic Han Chinese from Shandong,China so as to supplement the data of platelet donor bank in the region.Methods Peripheral blood samples of platelet donors from the region were genotyped for HPA-10 alleles by PCR-sequence specific primer(PCR-SSP)and direct sequencing.Results Among 1401 donors,a rare heterozygote carrier of HPA-10w(a+b+)was identified,which gave an allelic frequency of approximately 0.035%.Conclusion The detection of rare HPA-10bw antigen allele among ethnic Han Chinese from Shandong is useful for the diagnosis and prevention of neonatal alloimmune thrombocytopenia and post-transfusion purpura in the region.

关 键 词：HPA-10bw抗原 山东汉族人群 等位基因 PCR特异性引物分析法 

分 类 号：R394[医药卫生—医学遗传学]