响应面法优化高粱红色素提取工艺的研究  

Optimization of Extraction Process of Red Pigment from Sorghum by Response Surface Methodology

作　　者：米智 刘荔贞 武晓红 MI Zhi;LIU Li-zhen;WU Xiao-hong(College of Life Science,Shanxi Datong University,Datong 037009,China;College of Chemistry and Chemical Engineering,Shanxi Datong University,Datong 037009,China)

机构地区：[1]山西大同大学生命科学学院,山西大同037009 [2]山西大同大学化学与化工学院,山西大同037009

出　　处：《中国调味品》2022年第2期169-172,共4页China Condiment

基　　金：山西省应用基础研究计划(201901D211434);山西省高等学校科技创新计划(2021L387,2021L375);大同市农业重点研发计划项目(2021053,2021054);山西大同大学博士科研启动费(2014-B-13,2016-B-13)。

摘　　要：为了探究高粱红色素提取的最佳工艺条件,以高粱壳为材料,采用索氏提取法结合响应面试验,探究了乙醇浓度、料液比、提取时间和提取液pH 4个因素对高粱红色素吸光值的影响。结果显示:在乙醇浓度为65%、料液比为1∶8、提取时间为2 h和提取液pH为3的条件下,高粱红色素的吸光值最大,提取率约为25.2%,色价约为27.1。样品加标回收率为96.52%~104.86%,RSD为2.65%,该试验准确、可靠、稳定,为研究高粱红色素的提取工艺提供了参考依据。In order to explore the optimal technological conditions for the extraction of red pigment from sorghum,the effects of ethanol concentration,solid-liquid ratio,extraction time and pH value of extracting solution on the absorbance value of red pigment from sorghum are studied by Soxhlet extraction method combined with response surface test taking sorghum shell as the material.The results show that under the conditions of ethanol concentration of 65%,solid-liquid ratio of 1∶8,extraction time of 2 h and extraction solution pH value of 3,the absorbance value of red pigment from sorghum is the highest,the extraction yield is about 25.2%,and the color value is about 27.1.The recovery rate is 96.52%~104.86%and the RSD is 2.65%.The experiment is accurate,reliable and stable,which has provided references for the study on the extraction process of red pigment from sorghum.

关 键 词：高粱壳 红色素 色价 索氏提取 响应面试验 

分 类 号：TS202.3[轻工技术与工程—食品科学]