Effects of Pemphigus Vulgaris Serum on the Expression of ATP2C1 and PKP3 in HaCaT Cells  

作　　者：Qiao-Lin Pan Zhi-Min Xie Xiang-Nong Dai Yi Zhang Xu-Cheng Shen Qing-Qing Li Xing-Dong Ye 

机构地区：[1]Department of Dermatology,Institute of Dermatology,Guangzhou Medical University,Guangzhou,Guangdong 510095,China [2]Department of Dermatology,The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510799,China [3]Department of Dermatology,Guangzhou Institute of Dermatology,Guangzhou,Guangdong 510095,China

出　　处：《International Journal of Dermatology and Venereology》2021年第4期224-228,共5页国际皮肤性病学杂志（英文）

基　　金：The study was supported by Scientific Research Plan from Guangzhou Science and Technology Bureau(No.201904010352)。

摘　　要：Objective:To investigate the effects of serum from patients with pemphigus vulgaris(PV)on the transcription and protein expression level of calcium-transporting ATPase type 2C member 1(ATP2C1)and plakophilin 3(PKP3)in HaCaT cells.Methods:The HaCaT cells were divided into four groups:PV sera group,anti-Dsg3 monoclonal antibody group(AK23,positive control group),normal healthy serum group,and blank cell group.The groups were treated with corresponding different conditions for 24 hours.Quantitative polymerase chain reaction and Western blot were used to detect mRNA and protein levels of ATP2C1 and PKP3.Results:Compared with the blank group,the mRNA level of theATP2C1 and PKP3 genes in PV sera group was significantly increased by 384%and 404%,respectively(bothP<0.001).The treatment of PV sera and anti-Dsg3 antibody increased PKP3 protein expression(P=0.03 andP=0.004)but decreased protein expression of ATP2C1 in HaCaT cells(bothP<0.001).Conclusions:Our study indicates that serum from patients with PV promotes bothATP2C1 andPKP3 transcription in HaCaT cells,implying that the two genes may be involved in the pathological process of PV.

关 键 词：pemphigus vulgaris PATHOGENESIS ATP2C1 PKP3 DESMOGLEIN 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]