微波辅助酶法提取葛根黄酮的工艺优化及抗氧化活性研究  

Optimization of extraction process of Radix puerariae flavonoids by microwave-assisted enzymatic method and study on its antioxidant activity

作　　者：李少华[1] 高愿军 李翠翠[3] 秦令祥 LI Shao-Hua;GAO Yuan-Jun;LI Cui-Cui;QIN Ling-Xiang(Henan Polytechnic,Zhengzhou 450046,China;School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;School of Zhang Zhongjing Health Care and Food,Nanyang Institute of Technology,Nanyang 473000,China;Luohe Food Research Institute Co.,Ltd.,Luohe 462300,China)

机构地区：[1]河南职业技术学院,郑州450046 [2]郑州轻工业大学食品与生物工程学院,郑州450002 [3]南阳理工学院张仲景康养与食品学院,南阳473000 [4]漯河市食品研究院有限公司,漯河462300

出　　处：《食品安全质量检测学报》2022年第1期217-222,共6页Journal of Food Safety and Quality

基　　金：河南省科技攻关项目(212102110330)。

摘　　要：目的优化微波辅助酶法提取葛根黄酮的工艺,并对葛根黄酮的抗氧化活性进行评价。方法以葛根为原料,葛根黄酮得率为考核指标,在微波辅助、酶催化作用下,在单因素试验基础上,利用正交试验法对葛根黄酮提取工艺进行优化,并对葛根黄酮的抗氧化活性进行研究。结果最佳提取工艺参数为:复合酶添加量3.0%、酶解pH=6、微波功率200 W、提取时间40 min,在此条件下,葛根黄酮的得率为6.79%。葛根黄酮的抗氧化活性随着质量浓度的增加而增强,当葛根黄酮的质量浓度为200μg/mL时,葛根黄酮对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除率为80.06%、对羟自由基的清除率为73.28%。结论正交试验法优化了葛根黄酮的提取工艺,得到的葛根黄酮具有较强的抗氧化活性。Objective To optimize the extraction process of Radix puerariae flavonoids by microwave-assisted enzymatic method,and evaluate the antioxidant activity of flavonoids.Methods Taking Radix puerariae as raw material,the yield of Radix puerariae flavonoids as evaluation index,microwave-assisted,enzyme catalyzed,on the basis of single factor experiment,orthogonal test was used to optimize the extraction process of Radix puerariae flavonoids,and the antioxidant activity of Radix puerariae flavonoids was studied.Results The optimum extraction conditions were as follows:3.0% compound enzyme,pH=6,microwave power 200 W,extraction time 40 min.Under these conditions,the yield of Radix puerariae flavonoids was 6.79%.The antioxidant activity of Radix puerariae flavonoids increased with the increase of mass concentration,when the mass concentration of Radix puerariae flavonoids was 200μg/mL,the scavenging rates of Radix puerariae flavonoids on 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals and hydroxyl radicals were 80.06% and 73.28%.Conclusion The extraction process of Radix puerariae flavonoids is optimized by orthogonal test,and the obtained flavonoids have strong antioxidant activity.

关 键 词：葛根黄酮 微波 提取 抗氧化活性 

分 类 号：TQ28[化学工程—有机化工]