Maternal supply of cysteamine alleviates oxidative stress and enhances angiogenesis in porcine placenta  

作　　者：Shuangbo Huang Zifang Wu Zihao Huang Xiangyu Hao Longmiao Zhang Chengjun Hu Jianfu Wei Jinping Deng Chengquan Tan 

机构地区：[1]Guangdong Provincial Key Laboratory of Animal Nutrition Control,National Engineering Research Center for Breeding Swine Industry,Institute of Subtropical Animal Nutrition and Feed,College of Animal Science,South China Agricultural University,Guangzhou 510642,Guangdong,China [2]Guangzhou DaBeiNong Agri-animal Huabandry Science and Technology Co.,Ltd.,Guangzhou 510642,Guangdong,China [3]Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou 510642,Guangdong,China

出　　处：《Journal of Animal Science and Biotechnology》2022年第1期181-196,共16页畜牧与生物技术杂志（英文版）

基　　金：funded by Project of National Natural Science Foundation of China(No.31872985 and 31790411);Natural Science Foundation of Guangdong Province(No.2021A1515012116).

摘　　要：Background:Oxidative stress in placenta is associated with the occurrence of adverse pregnancy outcomes in sow,but there are few satisfactory treatment strategies for these conditions.This study investigated the potential of cysteamine(CS)as an antioxidant protectant for regulating the reproductive performance,redox status,and placental angiogenesis of sows.Methods:The placental oxidative stress status and vascular density of piglets with different birth weights:<1.0 kg(low birth weight,LBW)and 1.4–1.6 kg(normal birth weight,NBW)were evaluated,followed by allotting 84 sows to four treatments(n=21)and feeding them with a basal diet supplemented with 0,100,300,or 500 mg/kg of CS from d 85 of gestation to d 21 of lactation,respectively.Placenta,serum,and colostrum samples of sows or piglets were collected,and the characteristics of sows and piglets were recorded.Furthermore,the in vivo results were validated using porcine vascular endothelial cells(PVECs).Results:Compared with the NBW placentae,the LBW placentae showed increased oxidative damage and were vulnerable to angiogenesis impairment.Particularly,H2O2-induced oxidative stress prompted intracellular reactive oxygen species generation and inhibited the tube formation and migration of PVECs as well as the expression of vascular endothelial growth factor-A(VEGF-A)in vitro.However,dietary CS supplementation can alleviate oxidative stress and improve the reproductive performance of sows.Specifically,compared with the control group,dietary 100 mg/kg CS could(1)decrease the stillbirth and invalid rates,and increase both the piglet birth weight in the low yield sows and the placental efficiency;(2)increase glutathione and reduce malondialdehyde in both the serum and the colostrum of sows;(3)increase the levels of total antioxidant capacity and glutathione in LBW placentae;(4)increase the vascular density,the mRNA level of VEGF-A,and the immune-staining intensity of platelet endothelial cell adhesion molecule-1 in the LBW placentae.Furthermore,the in vitro experimen

关 键 词：ANGIOGENESIS CYSTEAMINE Oxidative stress PLACENTA SOW 

分 类 号：S828.3[农业科学—畜牧学]