Genome and transcriptome of Papaver somniferum Chinese landrace CHM indicates that massive genome expansion contributes to high benzylisoquinoline alkaloid biosynthesis  

作　　者：Li Pei Baishi Wang Jian Ye Xiaodi Hu Lihong Fu Kui Li Zhiyu Ni Zhenlong Wang Yujie Wei Luye Shi Ying Zhang Xue Bai Mengwan Jiang Shuhui Wang Chunling Ma Shujin Li Kaihui Liu Wanshui Li Bin Cong 

机构地区：[1]Institute of Forensic Science,Ministry of Public Security,No.17 South Muxidi Lane,Xicheng District,100038 Beijing,People’s Republic of China [2]College of Forensic Medicine,Hebei Medical University,Hebei Key Laboratory of Forensic Medicine,Innovation Center of Forensic Medical Molecular Identification,No.361 Zhongshan East Road,050017 Shijiazhuang,Hebei,People’s Republic of China [3]Novogene Bioinformatics Institute,Beijing,People’s Republic of China [4]Hebei University,No.180 Wusidong Road,Baoding,Hebei Province,People’s Republic of China [5]School of Life Sciences,Zhengzhou University,No.100 Science Road,450001 Zhengzhou,Henan,People’s Republic of China [6]Gansu Academy of Agri-Engineering Technology,No.234 Xinzhen Road,Huangyang Town,Liangzhou District,733006 Wuwei,Gansu,People’s Republic of China [7]Wuhan Botanical Garden,Chinese Academy of Sciences,430074 Wuhan,People’s Republic of China

出　　处：《Horticulture Research》2021年第1期38-50,共13页园艺研究（英文）

基　　金：the National Science Foundation of China(Grant 81671876);the Fundamental Research Funds for the Central Public Welfare Research Institutes(Grant 2016JB024)。

摘　　要：Opium poppy(Papaver somniferum)is a source of morphine,codeine,and semisynthetic derivatives,including oxycodone and naltrexone.Here,we report the de novo assembly and genomic analysis of P.somniferum traditional landrace‘Chinese Herbal Medicine’.Variations between the 2.62 Gb CHM genome and that of the previously sequenced high noscapine 1(HN1)variety were also explored.Among 79,668 protein-coding genes,we functionally annotated 88.9%,compared to 68.8%reported in the HN1 genome.Gene family and 4DTv comparative analyses with three other Papaveraceae species revealed that opium poppy underwent two whole-genome duplication(WGD)events.The first of these,in ancestral Ranunculales,expanded gene families related to characteristic secondary metabolite production and disease resistance.The more recent species-specific WGD mediated by transposable elements resulted in massive genome expansion.Genes carrying structural variations and large-effect variants associated with agronomically different phenotypes between CHM and HN1 that were identified through our transcriptomic comparison of multiple organs and developmental stages can enable the development of new varieties.These genomic and transcriptomic analyses will provide a valuable resource that informs future basic and agricultural studies of the opium poppy.

关 键 词：ALKALOID EXPANSION BENZYL 

分 类 号：S667.9[农业科学—果树学]