基于“高效薄层色谱法+”的食品分析技术:应用与展望  

Application and prospect of food analysis technology based on“high performance thin layer chromatography+”

作　　者：席兴军[1] 陈益胜 郑丹 侯华铭 黄彩虹 XI Xing-Jun;CHEN Yi-Sheng;ZHENG Dan;HOU Huaming;HUANG Cai-Hong(Institute of Agricultural Food Standardization,China National Institute of Standardization,Beijing 100191,China;College of Food Science and Engineering,Shanxi Agricultural University,Taiyuan 030801,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]中国标准化研究院农业食品标准化研究所,北京100191 [2]山西农业大学食品科学与工程学院,太原030801 [3]江南大学食品学院,无锡214122

出　　处：《食品安全质量检测学报》2021年第23期8980-8985,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基项目(21804058);山西省回国留学人员科研资助项目(2021-068);山西农业大学高层次人才专项(2021XG013);山西农业大学博士科研启动项目(2021BQ91)。

摘　　要：基于"高效薄层色谱法+"分析策略的检测方法已经成为分析化学领域一个新的热点。与传统柱色谱的闭环工作原理不同,高效薄层色谱法(high performance thin layer chromatography,HPTLC)是一个去中心化、开放的分析系统,分离结束后,分离结果停留在色谱板上而非废液瓶中。这一独特的优势使得HPTLC的分离结果可以方便地与很多无法与传统柱色谱系统兼容的检测手段实现无障碍融合,因此HPTLC不仅是一种高效的色谱工具,还可以作为多种离线检测方法高效集成融合的分析平台,在分析通量、简便性、精密度和适用范围等方面达到了理想的平衡,随着分析应用多样化的需求不断上升,多种检测技术手段融合的重要性日益凸显。因此HPTLC+有望成为新一代多功能一体化食品分析方法体系的基础。本研究综述了近期以HPTLC为平台的多种综合联用检测工具在食品分析领域的应用,并对其前景进行了展望。随着多个与联用检测相关的关键技术和设备的突破性进展,HPTLC实现了与几乎全部光谱、质谱和生物传感检测技术的联用全覆盖,因此具有极佳的发展前景。Analytical methods based on“high performance thin layer chromatography+”strategy has become a new hot spot in the field of analytical chemistry.Completely different from the closed loop working principle of traditional column chromatography,HPTLC is a decentralized and open analysis system,after the separation,all substances of the mixture stay on the chromatographic plate instead of the waste bottle.This featuring advantage enables HPTLC separation results to be easily linked to many detection methods that are not compatible to traditional column chromatography systems.Therefore,HPTLC is not only a effcicient chromatography tool,but also can be used as a multi-functionalized platform,striking an ideal balance between throughput,simplicity and flexibility.This reviewed article focused on the hyphenation of HPTLC with a large array of detectors,which might be promising tools for food analytical chemistry.With the growing demands of food analysis,the importance of the merge of multi-detector was rapidly increasing.Therefore,HPTLC-plus displayed great potential as the basis for the new generation of all-in-one food analytical methodology.

关 键 词：高效薄层色谱法 光密度 质谱 表面增强拉曼光谱 生物传感器 图像分析 

分 类 号：O657[理学—分析化学] TS207.3[理学—化学]