In situ growth of polydopamine on surface of covalent organic frameworks under the catalysis of acid phosphatase for dopamine detection  

作　　者：Fei Qu Ziwei Guo Dafeng Jiang Xian-En Zhao 

机构地区：[1]The Key Laboratory of Life-Organic Analysis,Qufu Normal University,Qufu 273165,China [2]The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine,Qufu Normal University,Qufu 273165,China [3]Department of Physical and Chemical Testing,Shandong Center for Disease Control and Prevention,Ji'nan 250014,China

出　　处：《Chinese Chemical Letters》2021年第11期3368-3371,共4页中国化学快报（英文版）

基　　金：supported by the Natural Science Foundation of Shandong Province,China(No.ZR2019QB010);National Natural Science Foundation of China(Nos.21705095,21775088);the Scientific Research Foundation of Qufu Normal University(No.BS D20130117).

摘　　要：Dopamine(DA)is easy to be oxidized and polymerizes to form polydopamine(pDA)in alkaline conditions,while the synthesis is usually time-consuming(48 h).Herein,the polymerization of DA is completed with 4 h under the catalysis of acid phosphatase(ACP).The high efficiency makes the detection of DA feasibility based on the self-polymerization of DA.In this assay,pDA is grown in situ on the surface of covalent organic frameworks(COFs),and then the fluorescence of COFs is quenched significantly.The linear range of DA is achieved from 0.5-50μmol/L with a detection limit of 0.16μmol/L.The detection of DA is not interfered with uric acid,ascorbic acid,and some phenolic compounds,because these substances cannot polymerize in the presence of ACP.Moreover,benefiting from the good sensitivity and selectivity,DA has been successfully determined by this strategy in human urine samples with satisfactory recoveries.

关 键 词：FLUORESCENCE ENZYME POLYDOPAMINE DOPAMINE Acid phosphatase 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]