OPN、Pyk2、p-AKT在舌鳞状细胞癌中的表达及临床意义  

Expressions and Clinical Significances of OPN,Pyk2 and p-AKT in Tongue Squamous Cell Carcinoma

作　　者：刘霞[1] 李强[2] 陈勇[2] 赵淑娟 郭荣珍 史炳霞 陈巧力[1] 南桂英[1] 孟召霞[1] 张冉[1] 张静[1] LIU Xia;LI Qiang;CHEN Yong;ZHAO Shu-juan;GUO Rong-zhen;SHI Bing-xia;CHEN Qiao-li;NAN Gui-ying;MENG Zhao-xia;ZHANG Ran;ZHANG Jing(Department of Clinical Medicine,Cangzhou Medical College,Cangzhou,Hebei 061001,China;Department of Oral and Maxillofacial Surgery,Cangzhou Central Hospital,Cangzhou,Hebei 061011,China)

机构地区：[1]沧州医学高等专科学校临床医学系,河北沧州061001 [2]沧州市中心医院口腔颌面外科,河北沧州061011

出　　处：《临床误诊误治》2022年第2期54-58,共5页Clinical Misdiagnosis & Mistherapy

基　　金：河北省医学科学研究课题计划项目(20211441)。

摘　　要：目的分析骨桥蛋白(OPN)、富含脯氨酸的酪氨酸激酶2(Pyk2)、磷酸化的丝氨酸/苏氨酸蛋白激酶(p-AKT)在舌鳞状细胞癌中的表达及临床意义。方法收集2018年2月—2020年2月诊治103例舌鳞状细胞癌组织(舌癌组)及距肿瘤边缘>0.5 cm的癌旁正常组织(对照组)。比较不同组织中OPN、Pyk2、p-AKT表达情况,分析OPN、Pyk2、p-AKT与舌鳞状细胞癌患者临床病理参数的关系,采用多因素Logistic回归分析影响舌鳞状细胞癌患者预后生存的危险因素。结果舌癌组OPN、Pyk2、p-AKT表达阳性率均高于对照组(P<0.01)。Ⅲ~Ⅳ期、高分化、有淋巴结转移舌鳞状细胞癌患者OPN、Pyk2、p-AKT表达阳性率分别高于Ⅰ~Ⅱ期、低-中分化、无淋巴结转移患者(P<0.05,P<0.01)。Ⅲ~Ⅳ期、高分化、有淋巴结转移及OPN、Pyk2、p-AKT表达阳性舌鳞状细胞癌患者病死率分别高于Ⅰ~Ⅱ期、低-中分化、无淋巴结转移及OPN、Pyk2、p-AKT表达阴性患者(P<0.05,P<0.01)。Ⅲ~Ⅳ期、高分化、有淋巴结转移以及OPN、Pyk2、p-AKT表达阳性是影响舌鳞状细胞癌患者预后生存的独立危险因素(P<0.01)。结论OPN、Pyk2、p-AKT在舌鳞状细胞癌组织中表达均明显上调,与癌细胞浸润和转移密切相关,可为舌鳞状细胞癌预后评估提供参考。Objective To analyze expressions and clinical significances of osteopontin(OPN),proline-rich tyrosine kinase 2(Pyk2)and phosphorylated serine/threonine protein kinase(p-AKT)in tongue squamous cell carcinoma(TSCC).Methods A total of 103 specimens of TSCC tissues(tongue cancer group)and normal tissues more than 0.5 cm from the edge of the tumor were collected(control group)from who had been diagnosed and treated between February 2018 and February 2020 were collected.Expressions of OPN,Pyk2,p-AKT in different tissues were compared,and correlations between OPN,Pyk2 and p-AKT protein with clinicopathological parameters of TSCC patients were analyzed.Multivariate logistic regression analysis was used to analyze risk factors affecting prognoses and survival conditions of patients with TSCC.Results The positive expression rates of OPN,Pyk2 and p-AKT in tongue cancer group were significantly higher than those in control group(P<0.01).Positive expression rates of OPN,Pyk2 and p-AKT in TSCC patients with stageⅢ-Ⅳ,high differentiation and lymph node metastasis were significantly higher than those in patients with stageⅠ-Ⅱ,low-medium differentiation and no lymph node metastasis(P<0.05,P<0.01).Mortality rates of TSCC patients with stageⅢ-Ⅳ,high differentiation,lymph node metastasis and positive expressed OPN,Pyk2 and p-Akt were significantly higher than those of patients with stageⅠ-Ⅱ,low-medium differentiation,no lymph node metastasis and negative expressed OPN,Pyk2 and p-Akt(P<0.05,P<0.01).StageⅢ-Ⅳ,high differentiation,lymph node metastasis,positive expressed OPN,Pyk2 and p-AKT were independent risk factors affected prognoses and survival conditions of TSCC patients(P<0.01).Conclusion Expressions of OPN,Pyk2 and p-AKT are significantly up-regulated in TSCC tissues,which are closely related to invasion and metastasis of cancer cells,and therefore they may provide references for prognosis evaluation of TSCC.

关 键 词：舌鳞状细胞癌 骨桥蛋白 富含脯氨酸的酪氨酸激酶2 磷酸化的丝氨酸/苏氨酸蛋白激酶 预后 危险因素 

分 类 号：R739.86[医药卫生—肿瘤]