响应面法优化子囊霉素发酵工艺  

Optimization in Fermentation Process for Ascomycin Production by Response Surface Methodology

作　　者：严凌斌[1] 张祝兰[1] 陈洲琴 张引[1] 邱观荣[1] 连云阳[1] YAN Lingbin;ZHANG Zhulan;CHEN Zhouqin;ZHANG Yin;QIU Guanrong;LIAN Yunyang(Fujian Provincial Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology,Fuzhou 350007,China)

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《化学与生物工程》2022年第2期32-36,共5页Chemistry & Bioengineering

基　　金：福建省属公益类研究所基本科研专项(2019R1005-2,2020R1005009)。

摘　　要：采用响应面法对子囊霉素的发酵培养基及发酵条件进行优化。通过Plackett-Burman设计(PBD)筛选影响子囊霉素产量的关键变量,通过最陡爬坡实验确定响应面分析中心点,再进一步通过中心组合设计(CCD)与响应面分析获得关键变量的最优值。结果表明,影响子囊霉素产量的关键变量糊精、黄豆粉和发酵时间的最优值分别为75 g·L^(-1)、28 g·L^(-1)、138 h,在此优化条件下子囊霉素产量可达743 mg·L^(-1),较优化前提高了46.8%。We optimized the fermentation medium and fermentation conditions of Ascomycin by response surface methodologies.Firstly,we screened the key variables affecting Ascomycin yield by Plackett-Burman design(PBD).Then,we determined the central point of response surface analysis by the steepest ascent experiment.Finally,we obtained the optimal value of key variables by central composite design(CCD)and response surface analysis.The results show that the optimal values of dextrin,soybean powder,and fermentation time are 75 g·L^(-1),28 g·L^(-1),and 138 h,respectively.Under above optimized conditions,the Ascomycin yield can reach 743 mg·L^(-1),which is 46.8%higher than that before optimization.

关 键 词：子囊霉素 响应面法 发酵培养基 发酵条件 吸水链霉菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ465.92