接骨木籽油挥发性成分及细胞毒性研究  

Volatile Components and Cytotoxicity of Sambucus Williamsii Seed Oil

作　　者：张伟国[1,2] 吕慧[3] 周兆丽 ZHANG Weiguo;LYU Hui;ZHOU Zhaoli(Shanghai Key Laboratory of Molecular Imaging,Shanghai University of Medicine and Health Sciences,Shanghai 201318,China;School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海健康医学院上海市分子影像学重点实验室,上海201318 [2]上海理工大学医疗器械与食品学院,上海200093 [3]上海海洋大学食品学院,上海201306

出　　处：《济南大学学报（自然科学版）》2022年第1期115-118,共4页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金项目(81803581);上海健康医学院协同创新重点专项(SPCI-18-18-003)。

摘　　要：采用气相色谱-质谱联用仪分析接骨木籽油中的挥发性成分,采用噻唑蓝比色法测定接骨木籽油对L02细胞的毒性。结果表明:接骨木籽油含有17种挥发性成分,其中醛类物质占挥发性成分总质量的81.57%;当接骨木籽油的质量浓度大于100 mg/L时,细胞毒性较大,L02细胞活度低于50%;接骨木籽油对L02细胞毒性的半数抑制浓度为94.8 mg/L,细胞毒性很低。Volatile components of Sambucus williamsii seed oil were analyzed by using gas chromatography-mass spectrometry,and cytotoxicity of Sambucus williamsii seed oil to L02 cells was determined through thiazolyl blue colorimetric method.The results show that there are 17 volatile components in Sambucus williamsii seed oil,in which aldehydes account for 81.57%of the total mass.When the mass concentration of Sambucus williamsii seed oil is greater than 100 mg/L,the cytotoxicity is greater,and the L02 cell activity is lower than 50%.The half inhibitory concentration of Sambucus williamsii seed oil on cytotoxicity of L02 cells is 94.8 mg/L,and the cytotoxicity is very low.

关 键 词：接骨木籽油 挥发性成分 细胞毒性 噻唑蓝比色法 气相色谱-质谱 

分 类 号：R282.1[医药卫生—中药学]