人工合成六倍体小麦耐盐种质资源的筛选及评价  

Screening and Evaluation of Salt-Tolerant Germplasm of Synthetic Hexaploid Wheat

作　　者：李小康 吴崇宁 王维[1] 李文淑 KISHII MASAHIRO 田纪春[1] 邓志英[1] LI Xiaokang;WU Chongning;WANG Wei;LI Wenshu;KISHII MASAHIRO;TIAN Jichun;DENG Zhiying(State Key Laboratory of Crop Biology,Key Laboratory of Crop Biology of Shandong Province,Group of Wheat Quality and Molecular Breeding,College of Agronomy,Shandong Agricultural University,Tai'an,Shandong 271000,China;Handan Academy of Agricultural Sciences,Handan,Hebei 056000,China;CIMMYT,Apdo.Postal 6-641,06600,Mexico,D.F.,Mexico.)

机构地区：[1]山东农业大学农学院,作物生物学国家重点实验室,小麦品质育种研究室,山东泰安271000 [2]邯郸市农业科学院,河北邯郸056000 [3]CIMMYT,Apdo.Postal6-641,06600,Mexico,D.F.,Mexico.

出　　处：《麦类作物学报》2021年第12期1487-1495,共9页Journal of Triticeae Crops

基　　金：山东省农业良种工程项目(No.2019LZGC01702);国家自然科学基金项目(31871613);国家重点研发计划项目(2016YFD0100502);山东省自然基金项目(ZR20171702170343)。

摘　　要：为从人工合成六倍体小麦中筛选出耐盐种质,对来自墨西哥玉米小麦改良中心(CIMMYT)的141份人工六倍体小麦材料,采用水培法,在小麦芽期和苗期进行不同浓度NaCl胁迫处理(0、100、150和200 mmol·L^(-1)),筛选适合耐盐性鉴定的盐胁迫浓度,并通过表型及生理指标测定、相关性分析、主成分分析、隶属函数分析、系统聚类等方法对人工合成六倍体小麦芽期和苗期的耐盐性进行综合评价。结果表明,盐胁迫对人工合成六倍体小麦的多数形态指标和生理指标均有不同程度的影响,根系干重和鲜重及根冠比受盐胁迫影响较大,对盐胁迫敏感性较强。100 mmol·L^(-1)NaCl为人工合成六倍体小麦耐盐性筛选的理想鉴定浓度。通过系统聚类将141份材料划分为四类,其中高耐盐材料17份(占12.06%),耐盐材料61份(占43.26%),中度耐盐材料48份(占34.04%),盐敏感材料15份(占10.64%)。In order to explore the salt tolerant germplasm of synthetic hexaploid wheat,141 samples of synthetic hexaploid wheat from CIMMYT were screened and evaluated.All samples were cultured in four different concentrations of NaCl solution(0 mmol·L^(-1),100 mmol·L^(-1),150 mmol·L^(-1),200 mmol·L^(-1))at germination stage and seedling stage,respectively.The salt tolerance of the germplasm of synthetic hexaploid wheat was evaluated by phenotype and physiological index determination,correlation analysis,principal component analysis,membership function value analysis and cluster analysis.The results indicated that most morphological and physiological indices of synthetic hexaploid wheat were differently affected by salt stress.Root dry weight,root fresh weight and the ratio of dry weight root to shoot were greatly affected,which were also very sensitive to salt stress.The ideal concentration of NaCl solution for screening salt tolerance of synthetic hexaploid wheat was 100 mmol·L^(-1),and the materials were divided into four groups:17(12.06%)materials with high salt tolerance,61(43.26%)materials with salt tolerance,48(34.04%)materials with moderate salt tolerance,and 15(10.64%)materials with salt sensitivity.

关 键 词：人工合成六倍体小麦 盐胁迫 综合评价 筛选 

分 类 号：S512.1[农业科学—作物学] S330