基于EST-SSR和种子特征的苇状羊茅遗传分析  

Genetic analysis of tall fescue based on EST-SSR markers and seed characteristics

作　　者：王子玥 刘凌云 刘曼[1] 常智慧[1] WANG Ziyue;LIU Lingyun;LIU Man;CHANG Zhihui(School of Grassland Science,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学草业与草原学院,北京100083

出　　处：《草业科学》2022年第1期66-74,共9页Pratacultural Science

基　　金：国家自然基金面上项目“褪黑素影响结缕草种子低温抗逆性分级机制研究”(31971770)。

摘　　要：苇状羊茅(Festuca arundinacea)是重要的冷季型草坪草和牧草,被广泛用于牧草生产、草坪绿化、生态修复等方面。本研究收集了36份不同来源苇状羊茅种质材料,利用表达序列标签-简单序列重复(EST-SSR)引物对材料进行遗传多样性分析,并结合种子表型特征,对它们的种质资源遗传多样性进行了综合评价与分析。结果表明:14对EST-SSR引物共扩增出140条带,多态条带总数为133条,多态带百分率95%;聚类结果具有一定的地域性趋势,但是不明显。当遗传距离在0.54时其聚类结果与种子特征的K均值聚类结果相似;欧洲群体的种子厚度和千粒重的变异系数最大,分别为25.02%和23.35%,其千粒重显著低于亚洲、北美洲群体(P<0.05)。多角度遗传分析表明36份材料具有高度异质性。本研究对苇状羊茅遗传资源的科学评价、核心种质创新和新品种选育具有重要意义。Tall fescue(Festuca arundinacea Schred)is an important cool-season turfgrass and forage grass that is widely used in forage production,turf greening,and ecological restoration.In this study,36 tall fescue germplasms from different sources were collected and analyzed for genetic diversity using EST-SSR primers and combined with seed phenotypic characteristics to comprehensively evaluate and analyze their germplasm diversity.The results showed that 14 pairs of EST-SSR primers amplified a total of 140 bands,and the total number of polymorphic bands was 133,with 95%polymorphic band percentage;the clustering results showed a certain geographical trend,but it was not obvious.In addition,when the genetic distance was 0.54,the clustering results were similar to the K-means clustering results of seed characteristics:the European population had the highest coefficient of variation for seed thickness and thousand seed weight,with values of 25.02%and 23.35%,respectively,and the thousand-grain weight of the European population was significantly lower than that of the Asian and North American populations(P<0.05).Multifaceted genetic analysis showed that the 36 materials were highly heterogeneous.Multifaceted genetic analysis is important for the scientific evaluation of tall fescue genetic resources,core germplasm innovation,and new variety selection.

关 键 词：苇状羊茅 种质资源 EST-SSR 种子表型 千粒重 UPGMA聚类 K均值聚类 

分 类 号：S688.4[农业科学—观赏园艺]