Short-form OPA1 is a molecular chaperone in mitochondrial intermembrane space  

作　　者：Deyang Yao Yukun Li Sheng Zeng Zhifan Li Zahir Shah Bigui Song Jinglei Liu Yi Wu Liang Yang Qi Long Wenqian Wang Zhijuan Hu Haite Tang Xingguo Liu 

机构地区：[1]School of Life Sciences,University of Science and Technology of China,Hefei 230027,China [2]Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),CAS Key Laboratory of Regenerative Biology,Joint School of Life Sciences,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou Medical University,Guangzhou 510530,China [3]Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,Institute for Stem Cell and Regeneration,Guangzhou Institutes of Biomedicine and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Guangzhou 510530,China [4]Centre for Regenerative Medicine and Health,Hong Kong Institute of Science&Innovation,Chinese Academy of Sciences,Hong Kong SAR,China

出　　处：《Science China(Life Sciences)》2022年第2期227-235,共9页中国科学（生命科学英文版）

基　　金：the National Key Re-search and Development Program of China(2017YFA0106300);the Stra-tegic Priority Research Program of the Chinese Academy of Sciences(XDA16030505);the National Key Research and Development Program of China(2017YFA0102900,2019YFA09004500,2017YFC1001602,2016YFA0100300,2018YFA0107100);the National Natural Science Foundation of China(32025010,31801168,31900614,31970709,81901275,32070729);the Key Research Program of Frontier Sciences,CAS(QYZDB-SSW-SMC001);International Cooperation Program,CAS(154144KYSB20200006);Guangdong Province Science and Technol-ogy Program(2020B1212060052,2018A030313825,2018GZR110103002,2020A1515011200,2020A1515010919,2020A1515011410,2021A1515012513);Guangzhou Science and Technology Program(201807010067,202002030277,202102021250,202102020827,202102080066);CAS Youth Innovation Promotion Association.

摘　　要：Mitochondria,double-membrane organelles,are known to participate in a variety of metabolic and signal transduction pathways.The intermembrane space(IMS)of mitochondria is proposed to subject to multiple damages emanating from the respiratory chain.The optic atrophy 1(OPA1),an important protein for mitochondrial fusion,is cleaved into soluble short-form(S-OPA1)under stresses.Here we report that S-OPA1 could function as a molecular chaperone in IMS.We purified the S-OPA1(amino acid sequence after OPA1 isoform 5 S1 site)protein and showed it protected substrate proteins from thermally and chemically induced aggregation and strengthened the thermotolerance of Escherichia coli(E.coli).We also showed that S-OPA1 conferred thermotolerance on IMS proteins,e.g.,neurolysin.The chaperone activity of S-OPA1 may be required for maintaining IMS homeostasis in mitochondria.

关 键 词：MITOCHONDRIA OPA1 CHAPERONE mitochondrial homeostasis heat shock 

分 类 号：Q244[生物学—细胞生物学]