银条菜粗多糖脱蛋白的方法研究  

Study of Deproteinization Method of Crude Polysaccharides from Stachys floridana Schuttl.ex Benth

作　　者：李洁[1] 陈琳 刘海棠[1,2] 刘锐[2] 朱铭强 LI Jie;CHEN Lin;LIU Haitang;LIU Rui;ZHU Mingqiang(China Light Industry Key Laboratory of Papermaking and Biorefinery,Tianjin Key Laboratory of Pulp and Paper,College of Light Industry Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;State Key Laboratory of Food Nutrition and Safety,College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;College of Mechanical and Electronic Engineering,Northwest A&F University,Yangling 712100,China)

机构地区：[1]中国轻工业造纸与生物质精炼重点实验室,天津市制浆造纸重点实验室,天津科技大学轻工科学与工程学院,天津300457 [2]食品营养与安全国家重点实验室,天津科技大学食品科学与工程学院,天津300457 [3]西北农林科技大学机械与电子工程学院,杨凌712100

出　　处：《天津科技大学学报》2022年第1期18-22,27,共6页Journal of Tianjin University of Science & Technology

基　　金：国家自然科学基金青年科学基金资助项目(31700516);食品营养与安全教育部重点实验室暨天津市食品营养与安全重点实验室开放课题(TJS202001)。

摘　　要：实验从银条菜中提取银条多糖,选用三氯乙酸法(TCA法)、盐析法以及盐酸法对银条菜进行脱蛋白处理,以多糖中蛋白去除率、多糖保留率以及糖醛酸保留率作为衡量指标优化脱蛋白最佳条件.实验结果表明:TCA法在三氯乙酸体积分数为5.00%时,蛋白去除率为97.25%,多糖保留率为86.89%,糖醛酸保留率为98.67%;盐析法中无水氯化钙添加量为0.30 g时,蛋白去除率为90.61%,多糖保留率为83.59%,糖醛酸保留率为88.67%;盐酸法调节pH为3时,蛋白去除率为95.92%,多糖保留率为86.31%,糖醛酸保留率为95.03%.TCA法是能够有效去除银条菜粗多糖中蛋白且多糖和糖醛酸保留较好的一种方法.In our experiment,the Stachys floridana Schuttl.ex Benth polysaccharide was extracted from the Stachys floridana Schuttl.ex Benth vegetable,and the trichloroacetic acid method(TCA method),the salting-out method and the hydrochloric acid method were used to deproteinize the Stachys floridana Schuttl.ex Benth vegetable.Protein removal rate,polysaccharide retention rate and uronic acid retention rate were used as the measurable indicators to optimize the best conditions for deproteinization.The results showed that when the volume fraction of TCA in the TCA method was 5.00%,the protein removal rate was 97.25%,the polysaccharide retention rate was 86.89%,and the uronic acid retention rate was 98.67%;the addition amount of anhydrous calcium chloride in the salting-out method was at 0.30g,the protein removal rate was 90.61%,the polysaccharide retention rate was 83.59%,and the uronic acid retention rate was 88.67%;when the pH was adjusted to 3 by the hydrochloric acid method,the protein removal rate was 95.92%,and the polysaccharide retention rate was 86.31%,and the retention rate of uronic acid was 95.03%.The results of our experiment indicate that the TCA method can effectively remove the protein in the polysaccharides of Stachys floridana Schuttl.ex Benth and retain the polysaccharides and uronic acid.

关 键 词：银条菜 多糖 脱蛋白 

分 类 号：TQ281[化学工程—有机化工]