Gene expression of granulosa and cumulus cells: The prospect inpredicting the quality and developmental competence of oocytesin vitro maturation  

作　　者：BICHUN ZHAO XUEQING WU YITONG YUAN YUANTAO GAO XIAO LI RUOCHEN DU SUMING XU RUXIN ZHANG CHUNFANG WANG 

机构地区：[1]Laboratory Animal Center,Shanxi Medical University,Taiyuan,030000,China [2]Center of Reproductive Medicine,Children’s Hospital of Shanxi and Women Health Center of Shanxi,Taiyuan,030000,China

出　　处：《BIOCELL》2020年第4期487-499,共13页生物细胞（英文）

基　　金：the Shanxi Province key research and development projects(No.201803D31068);Applied Basic Research Project of Shanxi Province(Nos.201801D121212,201901D211319,201901D111384);Science and Technology Innovation Project of Colleges and Universities in Shanxi Province(Nos.2019L0445,2019L0418).

摘　　要：In vitro maturation(IVM),a promising assisted reproductive technology(ART),has been evolving in clinical trials and applications.There is a huge potential demand for IVM in clinical practice because it reduces the stimulation of gonadotropins to patients and provides evidence for the safety of neonatal birth.Unfortunately,the maturation rate of oocytes in vitro is not as high as it is in vivo due to a different microenvironment.Moreover,there are still controversies in predicting the developmental capability of oocytes in IVM.The granulosa cells(GCs)and cumulus cells(CCs),closely surrounding the oocytes,play a critical role in oocytes development,while some studies have shown that they can reflect the quality of oocytes.Many studies have been conducted in terms of oocyte quality in transcriptional level in GCs and CCs of mice,Xenopus africanus,and Homo sapiens,which provides important enlightenment for the successful clinical application of IVM.However,no comprehensive reviews about how gene expression profiles affect oocytes quality have been reported.This review aimed to elucidate the gene expression profiles of GCs and CCs that have effects on the quality and developmental competence of oocytes maturation in vitro.And we also put forward a possible idea for ART in the future,integrating all gene expression profiles of GCs and CCs and predicting the quality of the oocytes.

关 键 词：Assisted REPRODUCTIVE technology Development In VITRO FERTILIZATION 

分 类 号：R73[医药卫生—肿瘤]