Decreased CD10-positive granulocytes for the differential diagnosis of myelodysplastic syndrome  

作　　者：JIYU WANG HUIPING WANG YING PAN QIANSHAN TAO ZHIMIN ZHAI 

机构地区：[1]Department of Hematology,The Second Hospital of Anhui Medical University,Hefei,230601,China

出　　处：《BIOCELL》2020年第4期607-611,共5页生物细胞（英文）

基　　金：the National Natural Science Foundation of China(81670179);Research Fund Project of Anhui Medical University(No.2018xkj026);National Natural Science Foundation Incubation Project of the Second Hospital of Anhui Medical University(Grant No.2019GQFY11).

摘　　要：Myelodysplastic syndromes(MDS)are highly heterogeneous myeloid neoplasms,and a large number of patients are difficult to diagnose and classify by blood and bone marrow examination.As a surface marker of granulocyte,studies have shown CD10 can be used to define the degree of granulocyte maturation in MDS patients.However,whether it can be used for differential diagnosis of MDS and other hematological diseases remains inconclusive.To explore the value of CD10 for differential diagnosis of MDS,60 newly diagnosed MDS,20 aplastic anemia(AA)patients,and 35 iron-deficient anemia(IDA)patients were selected for this study.Bone marrow(BM)specimens were processed for surface marker analysis and labeled with pre-conjugated monoclonal antibodies.Stained cells were detected by flow cytometry.Our results indicated that CD10-positive granulocytes were significantly decreased in BM of MDS patients than AA and IDA patients,and the level of CD10-positive mature granulocytes was not associated with the clinical stages of malignancy.Receiver operating characteristic(ROC)areas under the curve(AUC)of CD10-positive granulocytes was 0.86 and 0.85,respectively,in MDS patients than the IDA group and AA group with good specificity and sensitivity.Further,CD10-positive granulocytes were increased after effective treatment.In conclusion,we found the decrease in CD10-positive granulocytes has a differential diagnostic value of MDS.

关 键 词：MYELODYSPLASTIC SYNDROMES CD10 GRANULOCYTES Flow CYTOMETRY Differential diagnosis 

分 类 号：R55[医药卫生—血液循环系统疾病]