Exosomes derived from circBCRC-3-knockdown mesenchymal stemcells promoted macrophage polarization  

作　　者：QI SONG JUN ZHANG QIANG ZHANG JING LIU KE LV JIALU YAO YAFENG ZHOU 

机构地区：[1]Department of Cardiology,Suzhou Municipal Hospital Affiliated to Nanjing Medical University,Suzhou,215000,China [2]Department of Cardiology,Dushuhu Public Hospital Affiliated to Soochow University,Suzhou,215000,China [3]Department of Cardiology,The First Affiliated Hospital of Soochow University,Suzhou,215000,China

出　　处：《BIOCELL》2020年第4期623-629,共7页生物细胞（英文）

摘　　要：Macrophages play an essential role in the myocardial ischemia-reperfusion injury(MIRI),and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI.It has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage polarization.As circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182,this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage polarization.Firstly,circBCRC-3 was identified by divergent primers in mesenchymal stem cells(MSCs).Secondly,the exosome of MSCs was isolated and identified by transmission electron microscopy(TEM),nanoparticle-tracking analysis,and western blotting analysis.The expression level of circBCRC-3 in MSCexos was detected by RT-PCR.Finally,the polarization of the RAW264.7 cell phenotype was analyzed by flow cytometry.Moreover,we first identified circBCRC-3 in MSCs.The results further confirmed that MSCexo could effectively shift the macrophage polarization state from M1 towards the M2 phenotype,which indicated its role in MIRI cure.

关 键 词：EXOSOME CircBCRC-3 MESENCHYMAL stem cell Myocardial ISCHEMIA-REPERFUSION injury 

分 类 号：R73[医药卫生—肿瘤]