CHANGES IN NEUROPEPTIDES AFTER MUSIC EXPOSURE 429Cardioprotective effect of ivabradine via the AMPK/SIRT1/PGC-1αsignaling pathway in myocardial ischemia/reperfusion injuryinduced in H9c2 cell  

作　　者：XINGXING ZHU TIANFENG HUA MINGFEI WU JIATIAN WU JIANCHAO HONG MIN YANG 

机构地区：[1]The Second Affiliated Hospital of Anhui Medical University,Hefei,China [2]The Laboratory of Cardiopulmonary Resuscitation and Critical Care Medicine,The Second Affiliated Hospital of Anhui Medical University,Hefei,China [3]School of Pharmacy,Anhui Province Key Laboratory of Major Autoimmune Diseases,Anhui Medical University,Hefei,China

出　　处：《BIOCELL》2020年第3期431-441,共11页生物细胞（英文）

基　　金：the National Natural Science Foundation Youth Science Foundation(No.81601661);the Science Foundation for Post-doctoral researchers in Anhui Province of China(No.2016B140).

摘　　要：Post-resuscitation myocardial dysfunction(PRMD)is the most severe myocardial ischemia-reperfusion injury(MIRI)and is characterized by difficult treatment and poor prognosis.Research has shown the protective effects of the rational use of ivabradine(IVA)against PRMD,however,the molecular mechanisms of IVA remain unknown.In this study,an ischemia-reperfusion injury(IRI)model was established using hypoxic chambers.The results demonstrated that pretreatment with IVA reduced IRI-induced cytotoxicity and apoptosis.IVA attenuated mitochondrial damage,eliminated excess reactive oxygen species(ROS),suppressed IRI-induced ATP and NAD+,and increased the AMP/ATP ratio.We further found that IVA increased the mRNA levels of sirtuin 1(SIRT1)and peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α)and upregulated the expression levels of phosphorylated AMP-activated protein kinase(p-AMPK)/AMPK,SIRT1,and PGC-1αproteins.Interestingly,no change in AMPK mRNA levels was observed.Cardiomyocyte energy metabolism significantly changed after IRI.The aim of this study was to demonstrate the cardioprotective effect of Ivabradine via the AMPK/SIRT1/PGC-1αsignaling pathway in myocardial ischemia/reperfusion injury-induced in H9c2 cell.

关 键 词：IVABRADINE Myocardial ischemia REPERFUSION injury Energy metabolism Oxidative stress AMPK/SIRT1/PGC-1α pathway 

分 类 号：R28[医药卫生—中药学]