人体呼出挥发性有机物的口罩微萃取气相色谱-质谱分析  

Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling

作　　者：陈伟妮 袁子程 蔡深回 邹颖通 辛国斌[3] 狄丹丹 宋昕[4] 赵鹏 吴曼曼 胡斌 CHEN Wei-Ni;YUAN Zi-Cheng;CAI Shen-Hui;ZOU Ying-Tong;XIN Guo-Bin;DI Dan-Dan;SONG Xin;ZHAO Peng;WU Man-Man;HU Bin(Guangdong Provincial Engineering Research Center for Online Source Apportionment System of Air Pollution,Institute of Mass Spectrometry and Atmospheric Environment,Jinan University,Guangzhou 510632,China;Guangzhou Hexin Instrument Co.,Ltd,Guangzhou 510530,China;Key Laboratory of Forensic Toxicology,Ministry of Public Security,Beijing Municipal Public Security Bureau,Beijing 100192,China;Hefei Public Security Bureau,Hefei 230001,China;The Research Institute of Advanced Technologies,Ningbo University,Ningbo 315210,China)

机构地区：[1]暨南大学质谱仪器与大气环境研究所,广东省大气污染在线源解析系统工程技术研究中心,广州510632 [2]广州禾信仪器股份有限公司,广州510530 [3]法庭毒物分析公安部重点实验室(北京市公安局),北京100192 [4]合肥市公安局,合肥230001 [5]宁波大学高等技术研究院,宁波315210

出　　处：《分析化学》2022年第3期445-453,共9页Chinese Journal of Analytical Chemistry

摘　　要：人体呼气中含有大量与人类行为和健康状况有关的挥发性有机物(VOCs)。本研究采用口罩微萃取方法富集人体呼出VOCs,并联用3种气相色谱-质谱仪(GC-MS)对呼出VOCs进行分析鉴定与比对。优化了固相微萃取探针及富集采样时间;通过对比正常生理呼出VOCs和口罩的背景信号,探索了人体经过不同的食物摄入和口腔护理对呼出VOCs的影响,以及特征VOCs的可检测性和持续性;对比了常规、便携式和全二维G C-MS仪器对人体呼出VOCs的分析性能与特性。结果表明,口罩微萃取气相色谱-质谱方法可有效地检测口腔中大量的内源性和外源性挥发VOCs,并且具有采样安全简便、高效便捷、与不同仪器的适配性强等特点,为人体口腔健康与疾病诊断提供了新方法。Human exhaled breath contains a large number of volatile metabolites relating to human behavior and health states.In this study,solid phase microextraction(SPME)fibers were directly inserted into a facemask(SPME-in-facemask)to form a new facemask microextraction device for extracting human exhaled volatile organic compounds(VOCs).After sampling,SPME fibers were then coupled with different gas chromatography-mass spectrometers(GC-MS)for further identification of extracted VOCs,including conventional GC-MS,portable GC-MS and comprehensive two-dimensional GC-MS.Different SPME fibers and sampling time were investigated and compared.By comparing the background signals of face mask human exhaled VOCs,the effects of food intake and oral care on the compositions and changes of human exhaled VOCs were explored.The detectability and persistence of exhaled exogenous VOCs were investigated.The results showed that the facemask microextraction sampling coupled with GC-MS could be an efficient analytical tool for detecting a large amount of exhaled endogenous and exogenous VOCs.With the unique features of the facemask microextraction device,including safety,simplicity,convenient sampling,and strong adaptability to couple with different GC-MS methods in which portable GC-MS could provide a fast screening within minutes,while GC×GC-MS could give a comprehensive analysis for biomarker discovery.This work presented a novel promising strategy for investigating human health and oral diseases.

关 键 词：口罩 固相微萃取 人体呼气 气相色谱-质谱 挥发性有机物 

分 类 号：R446.1[医药卫生—诊断学] O657.63[医药卫生—临床医学]