CD133^(+)与CD133^(-)人原代胃癌细胞的差异表达基因及核心基因的筛选  

Screening of differentially expressed genes and core genes between CD133^(+)and CD133^(-)human primary gastric cancer cells

作　　者：贾岑岑 程薇 李雷蕾[1] 曾晓燕 廖永慧 谢渊[1] 周建奖[1] 赵艳[1] JIA Cencen;CHENG Wei;LI Leilei;ZENG Xiaoyan;LIAO Yonghui;XIE Yuan;ZHOU Jianjiang;ZHAO Yan(Key Laboratory of Endemic and Ethnic Diseases,Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou Province,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学地方病与少数民族疾病教育部重点实验室&贵州省医学分子生物学重点实验室,贵州贵阳550004

出　　处：《贵州医科大学学报》2022年第2期125-132,162,共9页Journal of Guizhou Medical University

基　　金：国家自然科学基金(31760328);贵州省自然科学基金(黔科合基础〔2020〕1Y333和黔科中引地〔2019〕4008)。

摘　　要：目的通过生物信息学方法筛选出CD133^(+)与CD133^(-)人原代胃癌细胞的差异表达基因(DEGs),寻找可能参与胃癌发生的关键基因。方法根据人CD133^(+)和CD133^(-)人原代胃癌细胞的转录组数据,以|log2FC|≥1,P<0.05为标准筛选DEGs;用Metascape对DEGs进行基因本体论(GO)富集及京都基因和基因组数据库(KEGG)通路富集分析,利用STRING数据库及Cytoscape 3.8.0软件构建蛋白质^(-)蛋白质相互作用(PPI)网络,筛选核心基因;利用肿瘤基因组图谱(TCGA)数据库分析核心基因与胃癌患者总生存率的关系。结果在CD133^(+)与CD133^(-)人原代胃癌细胞间筛选出305个DEGs,其中下调的DEGs 227个,上调的DEGs 78个;这些DEGs主要参与钙离子通道调节、DNA复制及DNA的代谢过程;KEGG通路分析提示DEGs主要富集于IL^(-)17信号通路、RNA运输以及MAPK信号通路;PPI筛选出20个关键基因,其中趋化因子8(CXCL8)、TCP1伴侣蛋白亚基2(CCT2)、核糖体产物因子2(RPF2)、蛋白酶体20S亚基α4(PSMA4)、胞质伴侣素6A(CCT6A)、蛋白酶体26S亚基(PSMD12)以及凝聚素Ⅰ复合物亚基G(NCAPG)表达与胃癌患者的总生存率负相关,RUNX家族转录因子2表达与胃癌患者的总生存率正相关(P<0.05)。结论获得CD133^(+)与CD133^(-)人原代胃癌细胞的305个DEGs及20个核心基因。Objective To find the key genes that may be involved in the occurrence of gastric cancer by screening the differentially expressed genes(DEGs)between CD133^(+) and CD133^(-) human primary gastric cancer cells using bioinformatics methods.Methods Based on the transcriptome data between CD133^(+) and CD133^(-) human primary gastric cancer cells obtained in our previous research,DEGs were screened according to the criteria of|log2FC|≥1,P<0.05.Gene Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment of DEGs were analyzed by Metascape.The STRING database and Cytoscape 3.8.0 software were used to construct a protein-protein interaction(PPI)network and screen the core genes.The relationship between core genes and overall survival rate of patients with gastric cancer was analyzed by using tumor Genome Atlas(TCGA)database.Results A total of 305 DEGs were screened between CD133^(+) and CD133^(-) human primary gastric cancer cells,including 227 down-regulated DEGs and 78 up-regulated DEGs.These DEGs are mainly involved in calcium channel regulation,DNA replication,and DNA metabolism.The KEGG pathway analysis showed that these DEGs were mainly enriched in IL-17 signaling pathway,RNA transport,and MAPK signaling pathway.The PPI network analysis identified 20 key genes,in which the expression of CXCL8,CCT2,RPF2,PSMA4,CCT6A,PSMD12,and NCAPG genes were negatively related to the overall survival rate of gastric cancer patients,while RUNX2 gene expression was positively related to the prognosis of gastric cancer patients(P<0.05).Conclusion Three hundred and five DEGs and twenty core genes of CD133^(+) and CD133^(-) human primary gastric cancer cells are obtained.

关 键 词：原代胃癌细胞 CD133 RNA-SEQ 差异表达基因 生物信息学 信号通路 

分 类 号：R735.2[医药卫生—肿瘤]