法夫酵母类胡萝卜素对UVA氧化损伤HSF细胞的保护作用  

Protective Effects on HSF Cells under the UVA Oxidative Damage by Phaffia rhodozyma Carotenoids

作　　者：陈晓晨 白曼利 陈昭华[1] 杜希萍[1,2,3,4] CHEN Xiaochen;BAI Manli;CHEN Zhaohua;DU Xiping(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,China;Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed,Xiamen,Southern Ocean Technology Center of China,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,厦门361021 [2]福建省食品微生物与酶工程重点实验室,厦门361021 [3]厦门市食品与生物工程技术研究中心,厦门361021 [4]厦门南方海洋研究中心海藻资源化利用与深加工重点实验室,厦门361021

出　　处：《激光生物学报》2022年第1期61-69,共9页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(32001672,31501448);福建省海洋经济发展补助资金项目(FJHJF-L-2020-1);福建省自然科学基金项目(2021J01833)。

摘　　要：本文将法夫酵母虾青素、β-胡萝卜素、海胆酮和三者的组合物作用于长波长紫外线(UVA)氧化损伤的人皮肤成纤维(HSF)细胞,以乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量为指标评价类胡萝卜素对HSF细胞因紫外辐射而氧化损伤的保护作用。结果表明:单一类胡萝卜素及它们的组合均能显著降低LDH活性和MDA含量(P<0.05),对UVA导致的HSF细胞的氧化损伤具有保护作用;相同质量浓度下,虾青素降低LDH活性的能力优于β-胡萝卜素,对HSF细胞氧化损伤的保护效果较好。本研究为利用类胡萝卜素开发对UVA辐射氧化损伤具有保护作用的化妆品和保健品提供了理论依据。In this paper, the results showed that single carotenoid and their combinations significantly reduced LDH activity and MDA content(P<0.05), and had protective effects on UVA-induced oxidative damage to HSF cells. At the same concentration, astaxanthin had better LDH reduction activity than β-carotene, and had better protective effect on oxidative damage of HSF cells. This study provides a theoretical basis for the development of cosmetics and health care products with the protective effect on UVA radiation oxidative damage with carotenoids.

关 键 词：虾青素 Β-胡萝卜素 海胆酮 紫外辐射 氧化损伤 

分 类 号：R285[医药卫生—中药学]