表面活性剂辅助超声提取柿叶总黄酮工艺优化  

Optimization of Surfactant-assisted Ultrasonic Extraction of Total Flavonoids from Persimmon Leaves

作　　者：张富坤 孙媛 高艺菲 王娇 高世杰[2] 赵盼 柯仲成[3] 赵东升 ZHANG Fukun;SUN Yuan;GAO Yifei;WANG Jiao;GAO Shijie;ZHAO Pan;KE Zhongcheng;ZHAO Dongsheng(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong University of Traditional Chinese Medicine Experimental Center,Jinan 250355,China;College of Chemistry and Chemical Engineering,Huangshan University,Huangshan 245041,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学实验中心,山东济南250355 [3]黄山学院化学化工学院,安徽黄山245041

出　　处：《食品工业科技》2022年第5期224-229,共6页Science and Technology of Food Industry

基　　金：山东省大学生创新创业训练计划项目(S202010441014);山东省中医药科技发展计划项目(2019-0030);国家自然科学基金项目(81903780);山东中医药大学中药资源保护与质量评价青创团队支持项目。

摘　　要：目的:优选表面活性剂辅助超声提取柿叶总黄酮工艺。方法:以柿叶为材料,总黄酮得率和DPPH自由基清除率为指标,分别考察表面活性剂种类、表面活性剂质量分数、液料比、超声时间及超声温度对指标的影响,并通过Box-Behnken设计-双响应面法优选总黄酮提取工艺。结果:最佳提取工艺:乙醇体积分数70%、十二烷基硫酸钠质量分数0.6%、液料比22:1 mL/g、超声温度30℃和超声时间7.3 min,此时柿叶总黄酮得率的实际值为(103.63±0.10)mg/g,DPPH自由基清除率为(65.35%±0.12%)。结论:优化后的提取工艺可有效地提高柿叶总黄酮得率,为柿叶黄酮类成分资源的研究开发与利用提供一定的理论基础。Objective:The surfactant-assisted ultrasonic extraction of total flavonoids from persimmon leaves was optimized.Methods:With the extraction rate of total flavonoids and DPPH radical scavenging rate as the double-indicator response values,the effects of surfactant type and mass fraction,liquid-to-material ratio,ultrasonic time,and ultrasonic temperature were investigated,and the Box-Behnken design-double response surface methodology was used to optimize the extraction process of total flavonoids from persimmon leaves.Results:The obtained optimal extraction process was as follows:Ethanol volume fraction of 70%,the mass fraction of sodium dodecyl sulfate 0.6%,liquid-to-material ratio of22:1 mL/g,ultrasonic temperature of 30 ℃ and time of 7.3 min.Under these conditions,the actual extraction rate of total flavonoids from persimmon leaves was(103.63±0.10) mg/g and the DPPH radical scavenging rate was(65.35%±0.12%).Conclusion:The optimized extraction process can effectively improve the yield of total flavonoids from persimmon leaves and provide a theoretical basis for the development and utilization of flavonoids resources from persimmon leaves.

关 键 词：柿叶 总黄酮 抗氧化活性 表面活性剂 超声提取 双响应面法 

分 类 号：R284[医药卫生—中药学]