高效液相色谱法同时检测液体食品中6种人工合成色素  

Simultaneous Detection of Six Synthetic Pigments in Liquid Food by High Performance Liquid Chromatography

作　　者：童优芸 王小宝 王晓燕 丁文慧 TONG You-yun;WANG Xiao-bao;WANG Xiao-yan(Sion Inspection Service Co.,Ltd.,Ningbo,Zhejiang 315000;Ningbo Academy of Product and Food Quality Inspection(Ningbo Fibre Inspection Institute),Ningbo,Zhejiang 315000)

机构地区：[1]诺安检验服务有限公司,浙江宁波315000 [2]宁波市产品食品质量检验研究院(宁波市纤维检验),浙江宁波315000

出　　处：《安徽农业科学》2022年第4期190-194,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]通过优化高效液相色谱条件和前处理步骤,建立高效液相色谱法同时测定液体食品中柠檬黄、苋菜红、胭脂红、日落黄、亮蓝、诱惑红6种人工合成色素,以此提高合成色素的检测效率。[方法]试验优化了前处理方法,液体食品基质比较干净时采用直接水提进样法,基质比较复杂时采用固相萃取净化法;优化了梯度洗脱程序和波长程序等仪器条件。[结果]6种人工合成色素浓度在5~50μg/mL线性关系良好(R^(2)>0.999);柠檬黄、苋菜红、日落黄、胭脂红、诱惑红的检出限为0.03125 mg/kg,亮蓝的检出限为0.12500 mg/kg,样品添加标准物质后平均回收率在89.0%~100.3%,相对标准偏差(RSD)在1.56%~3.12%。[结论]该研究可为实验室日常的人工合成色素检测提供参考。[Objective]By optimizing the conditions and pretreatment steps of high performance liquid chromatography,a high performance liquid chromatography method was established for the simultaneous determination of 6 synthetic pigments of lemon yellow,amaranth,carmine,sunset yellow,brilliant blue,and allure red in liquid food,so as to improve the detection efficiency of synthetic pigments.[Method]The experiment optimized the pretreatment method.The direct water extraction method was used when the liquid food matrix was relatively clean,and the solid phase extraction purification method was used when the matrix was more complicated;the instrument conditions such as gradient elution procedure and wavelength procedure were optimized.[Result]The concentration of 6 kinds of synthetic pigments has a good linear relationship between 5-50μg/mL(R^(2)>0.999).The detection limit of lemon yellow,amaranth,sunset yellow,carmine and allure red was 0.03125 mg/kg,and the detection limit of brilliant blue was 0.12500 mg/kg.The average recovery rate of samples after adding standard substances was 89.0%-100.3%,the relative standard deviation(RSD)was 1.56%-3.12%.[Conclusion]This study can provide reference for the daily synthetic pigment detection in laboratories.

关 键 词：高效液相色谱 食品 人工合成色素 仪器条件 前处理 方法优化 

分 类 号：TS207[轻工技术与工程—食品科学]