解淀粉芽孢杆菌分子遗传操作及其应用研究进展  

Research Progress in Molecular Genetic Manipulation Technology of Bacillus amyloliquefaciens and Its Application

作　　者：邱益彬 马艳琴 沙媛媛 朱逸凡 苏二正 雷鹏[2] 李莎[2] 徐虹[2] QIU Yi-bin;MA Yan-qin;SHA Yuan-yuan;ZHU Yi-fan;SU Er-zheng;LEI Peng;LI Sha;XU Hong(College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing 210037;College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211816;Yangzhou Rixing Biotechnology Limited Liability Company,Yangzhou 225601)

机构地区：[1]南京林业大学轻工与食品学院,南京210037 [2]南京工业大学食品与轻工学院,南京211816 [3]扬州日兴生物科技股份有限公司,扬州225601

出　　处：《生物技术通报》2022年第2期205-217,共13页Biotechnology Bulletin

基　　金：国家自然科学基金项目(22008122);江苏省高等学校自然科学研究项目(20KJB530018);生物基材料与绿色造纸国家重点实验室开放基金(GZKF202013)。

摘　　要：解淀粉芽孢杆菌是FDA认定的安全级(generally recognized as safe,GRAS)菌株,在工业酶制剂、高分子聚合物、大宗化学品、绿色生物农药等方面的生产具有突出的优势。近年来,随着解淀粉芽孢杆菌的分子遗传操作技术越来越成熟,对利用该菌开发成微生物发酵平台化菌株用于合成生物学制造领域提出了更迫切的需求。文中围绕解淀粉芽孢杆菌的遗传操作工具、代谢改造应用和未来发展前景等方面进行了详细综述,为进一步推动解淀粉芽孢杆菌合成生物学技术的创新与发展提供借鉴和参考。Bacillus amyloliquefaciens is a Generally Recognized as Safe(GRAS)strain recognized by the FDA.It has outstanding advantages in the production of industrial enzyme preparations,high molecular polymers,bulk chemicals,and green biological pesticides.In recent years,as the molecular genetic manipulation technology of B.amyloliquefaciens has become more and more mature,there has been a more urgent need for the use of the bacteria to develop microbial fermentation platform strains for use in the field of synthetic biology manufacturing.In this paper,the genetic manipulation tools,metabolic modification applications and future development prospects of B.amyloliquefaciens are reviewed in detail,which will provide reference for further promoting the innovation and development of B.amyloliquefaciens on synthetic biology technology.

关 键 词：解淀粉芽孢杆菌 底盘细胞 分子遗传操作 遗传改造 代谢工程 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]