氟苯尼考在北京油鸡体内残留变化规律研究  

Study on Regularity of Residual Change of Florfenicol in Beijing Fatty Chicken

作　　者：米璐 甘芝霖 王雪[2] 张星联[2] 杨曙明[2] 邱静[2] 徐贞贞[2] MI Lu;GAN Zhilin;WANG Xue;ZHANG Xinglian;YANG Shuming;QIU Jing;XU Zhenzhen(College of Food Science and Nutritional Engineering/National Engineering Research Center of Fruit and Vegetable Processing/Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing 100083, China;Institute of Quality Standard & Testing Technology for Agro-Products/Key Laboratory of Agro-Food Safety and Quality, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100081, China;College of Biological Sciences and Biotechnology/Beijing Key Laboratory of Forestry Food Processing and Security, Beijing Forestry University, Beijing 100083, China)

机构地区：[1]中国农业大学食品科学与营养工程学院/国家果蔬加工工程技术研究中心/农业农村部果蔬加工重点实验室/食品非热加工北京市重点实验室,北京100083 [2]中国农业科学院农业质量标准与检测技术研究所/农业农村部农产品质量安全重点实验室,北京100081 [3]北京林业大学生物科学与技术学院/林业食品加工与安全北京市重点实验室,北京100083

出　　处：《食品科学技术学报》2022年第1期132-139,共8页Journal of Food Science and Technology

基　　金：2019年国家农产品质量安全风险评估计划项目(GJFP2019025)。

摘　　要：为研究氟苯尼考在北京油鸡体内的残留变化规律,挑选160只健康的北京油鸡,随机分为4组,给药组公母各40只,对照组公鸡40只,母鸡33只(40只母鸡中7只在试验中死亡)。给药组以公鸡21.6 mg/kg(以体质量计)和母鸡21.4 mg/kg(以体质量计)的剂量,每隔24 h给药1次,连续5 d口服饲喂氟苯尼考粉,对照组不给任何抗菌药物,与给药组同环境饲养。于停药后第2.5 h~21 d时各屠宰6只公鸡(给药组4只、对照组2只)和6只母鸡(给药组4只、对照组2只),分别采集肉、肾、肝样本,经超高效液相色谱串联三重四级杆质谱法测定组织中氟苯尼考和氟苯尼考胺的残留量,并采用WT 1.4软件推算休药期。结果表明,氟苯尼考和氟苯尼考胺在肝脏组织中的平均残留量最高,可达(881.88±278.90)μg/kg,之后依次是肾脏和肌肉组织,药物消除较慢。为保证食品安全,推荐氟苯尼考在北京油鸡体内的休药期不低于12 d。To study the regularity of residual change of florfenicol in Beijing fatty chicken,153 healthy Beijing fatty chickens were randomly divided into 4 groups,40 cocks and 40 hens in the treatment group,40 cocks and 33 hens in the control group(7 hens died during the experiment).The daily dose of the drug for treatment group was 21.6 mg/(kg·bw)for cocks and 21.4 mg/(kg·bw)for hens.The treatment group was fed with florfenicol powder orally for 5 consecutive days.The control group fed without any antibacterial drug was kept under the same condition as the treatment group.At 2.5 h-21 d after drug withdrawal,6 cocks(4 in the treatment group and 2 in the control group)and 6 hens(4 in the treatment group and 2 in the control group)were slaughtered.The samples of muscle,kidney and liver were collected,respectively.The residues of florfenicol and florfenicol-amine in tissues were determined by ultra-performance liquid chromatography-tandem mass spectrometry,and the withdrawal period was calculated by WT 1.4 software.The results showed that the average residue of florfenicol and florfenicol-amine with the value of(881.88±278.90)μg/kg in liver was the highest,which was followed by values in kidney and muscle tissue.And the drug was eliminated slowly.In order to ensure food safety,it recommended that the withdrawal period of florfenicol in Beijing fatty chicken should be no less than 12 days.

关 键 词：北京油鸡 氟苯尼考 氟苯尼考胺 残留变化 休药期 

分 类 号：TS207.5[轻工技术与工程—食品科学] TS251.7[轻工技术与工程—食品科学与工程]