转基因玉米深加工产品中DNA提取方法的研究  

Study on DNA Extraction Method in Transgenic Maize Products

作　　者：王辉 赵爱君 张全军[3] WANG Hui;ZHAO Aijun;ZHANG Quanjun(Dongying Customs of the People's Republic of China,Dongying,Shandong 257091,China;Shandong Jilong Group Co.,Ltd.,Laiyang,Shandong 265200,China;Weifang Business Vocational College,Weifang,Shandong 262234,China)

机构地区：[1]中华人民共和国东营海关,山东东营257091 [2]山东吉龙集团有限公司,山东莱阳265200 [3]潍坊工商职业学院,山东潍坊262234

出　　处：《农产品加工》2022年第3期38-41,共4页Farm Products Processing

摘　　要：对转基因玉米深加工产品中DNA的提取技术进行了研究。首先,通过经典CTAB法、CTAB初步改良法、CTAB有效改良法和煮沸法等4种方法提取了玉米片和爆米花中的DNA;其次,用琼脂糖电泳检测法将DNA进行了分离;再次,用紫外可见分光光度法检测出4种方法提取的DNA的纯度。结果表明,4种方法中的CTAB有效改良法提取DNA的完整性、纯度更好。In this paper,we researched the extraction technology of DNA from transgenic maize. First,extracted DNA from corn flakes and popcorn by classical CTAB、 Preliminary improvemented CTAB、 improved CTAB method and the boiling method;Second,we separated DNA by agarose gel electrophoresis. Third,we checked the purity of the DNA. The experimental results showed that improved CTAB was the best method in the integrality and purity.

关 键 词：转基因玉米 DNA提取方法 玉米片 爆米花 

分 类 号：R155.5[医药卫生—营养与食品卫生学]