光谱法研究赤藓红和溶菌酶的相互作用  

Study on the Interaction Between Erythrosine and Lysozyme by Spectrometry

作　　者：张彦青[1] 刘娜[1] 张丽[1] 付凤艳 张彦[1] 陈灵智[1] 王晓红 ZHANG Yan-qing;LIU Na;ZHANG Li;FU Feng-yan;ZHANG Yan;CHEN Ling-zhi;WANG Xiao-hong(Department of Applied Chemistry,Hengshui University,Hengshui 053000,China)

机构地区：[1]衡水学院应用化学系,河北衡水053000

出　　处：《中国调味品》2022年第3期187-191,共5页China Condiment

基　　金：河北省高等学校科学技术研究项目(Z2020133);衡水市科技计划指令项目(2020014013C)。

摘　　要：文章通过光谱法和分子对接技术,研究了赤藓红与溶菌酶分子之间的相互作用。研究结果表明赤藓红能够以静态猝灭形式有效地猝灭溶菌酶的荧光,形成1∶1复合物。热力学结果表明赤藓红与溶菌酶体系主要作用力类型为疏水作用力和氢键,分子对接的结果也进一步说明了这个观点。根据荧光实验数据建立了赤藓红与溶菌酶的结合率模型,结果表明,随着温度的升高,无论是蛋白结合率还是色素结合率都呈现下降的趋势,结合体系的稳定性越来越低,并且由分子对接结果得知结合作用会对溶菌酶的活性产生影响。The interaction between erythrosine and lysozyme is studied by spectrometry and molecular docking technology.The results show that erythrosine could effectively quench the fluorescence of lysozyme in the form of static quenching and form a 1∶1 complex.The thermodynamic results show that the main types of interaction between erythrosine and lysozyme system are hydrophobic interaction and hydrogen bond,and the results of molecular docking further illustrate this view.According to the fluorescence experimental data,the binding rate model of erythrosine and lysozyme is established.The results show that with the increase of temperature,both protein binding rate and pigment binding rate show a downward trend,the stability of the binding system becomes lower and lower,and it is known from the molecular docking results that the binding effect would affect the activity of lysozyme.

关 键 词：赤藓红 溶菌酶 光谱法 相互作用 结合率 

分 类 号：TS202.3[轻工技术与工程—食品科学]