高效液相色谱法测定食品中番泻苷A和番泻苷B的含量  

Determination of Sennoside A and Sennoside B in Foods by High-Performance Liquid Chromatography

作　　者：张志刚 袁文萱 吴敏 郑向华 方恩华 吴娇敏 陈晨 王东 ZHANG Zhigang;YUAN Wenxuan;WU Min;ZHENG Xianghua;FANG Enhua;WU Jiaomin;CHEN Chen;WANG Dong(Xiamen Customs Technical Center,Xiamen 361013,China)

机构地区：[1]厦门海关技术中心,福建厦门361013

出　　处：《食品科技》2022年第1期319-323,共5页Food Science and Technology

基　　金：福建省社会发展引导性(重点)项目(2018Y0075);国家重点研发计划项目(2017YFC1601606)。

摘　　要：建立高效液相色谱法测定食品中番泻苷A和番泻苷B的方法。样品经过0.2%碳酸氢钠溶液超声提取,采用Agilent ZORBAX SB-C;(4.6 mm×150 mm,5.0 μm)色谱柱分离,以四氢呋喃-水-乙酸为流动相洗脱,DAD检测器,检测波长:340 nm。番泻苷A、番泻苷B在1~100 μg/mL范围线性关系良好,相关系数均大于0.999,检出限(S/N=3)为15 mg/kg,定量限(S/N≥10)为50 mg/kg。以空白样品进行添加回收试验,番泻苷A的回收率为80.88%~96.34%,相对标准偏差(Relative Standard Deviation)为3.07%~7.73%,番泻苷B的回收率为81.32%~102.2%,相对标准偏差(RSD)为2.62%~9.62%。该方法前处理简单、灵敏度高、操作简便、准确性良好。在市售的果冻类食品中,检出阳性样品。To establish a method for the determination residues of sennoside A and sennoside B in foods by High-performance liquid chromatography (HPLC).The samples were extracted by ultrasonic with 0.2% sodium bicarbonate solution,Agilent Zorbax SB-C;(4.6 mm×150 mm,5.0 μm) chromatographic column was used for the separation,eluted by tetrahydrofuran water acetic acid as mobile phasephase,detected at 340 nm by diode arraydetector (DAD).The calibration curves showed a good linearity in the range of 1~100 μg/mL for sennoside A and sennoside B for perchlorate with r≥0.999.The limit detections of sennoside A and sennoside B (S/N>3) were 15 mg/kg.And the limit quantitatives of sennoside A and sennoside B (S/N>10) were 50 mg/kg.The average recoveries of sennoside A were between 80.88% to 96.34%.The relative standard deviations (RSD) were 3.07%~7.73%.The average recoveries of sennoside B were between 81.32% to 102.2%.The relative standard deviations (RSD) were 2.26%~9.62%.The method is simple,sensitive and accurate.The positive samples were detected in jelly foods on the market.

关 键 词：番泻苷A 番泻苷B 残留量 高效液相色谱法 食品 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]