新疆伊吾县野山杏多肽对衰老模型小鼠的抗氧化作用  

Antioxidant Effect of Peptides of Wild Apricot from Yiwu County in Xinjiang in Aging Mouse Model

作　　者：蒋丽 卢悦 买迪娜木·阿布力米提 商雪珂 许贺志祥 杨晓君 JIANG Li;LU Yue;Maidenamu·Ablimiti;SHANG Xueke;XU Hezhixiang;YANG Xiaojun(Sinopharm Xinjiang Xinte Pharmaceutical Co.Ltd.,Urumqi 830000,China;College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Key Laboratory of Postharvest Science and Technology of Fruit,Urumqi 830052,China)

机构地区：[1]国药集团新疆新特药业有限公司,新疆乌鲁木齐830000 [2]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [3]新疆果品采后科学与技术重点实验室,新疆乌鲁木齐830052

出　　处：《现代食品科技》2022年第2期46-55,11,共11页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(31460393)。

摘　　要：采用酶解法提取新疆伊吾县野山杏多肽,探究其对衰老模型小鼠的抗氧化作用。通过单因素实验,考察酶底物比、料液比、提取时间、pH值和酶解温度对野山杏多肽水解度的影响,结合正交试验,优化野山杏多肽提取工艺;以提取物为原料,D-半乳糖制备小鼠衰老模型,分为空白对照组,野山杏多肽低、中、高剂量组(50、75、100 mg/kg),衰老模型组以及Vc阳性对照组,连续灌胃饲养30 d,测定血清、肝组织和脑组织匀浆中GSH-Px、SOD活力和MDA含量。酶解法提取野山杏多肽的最佳工艺:酶底物比为4%,酶解时间120 min,pH值为8,酶解温度为65℃,料液比为1:25,多肽水解度为35.73%,多肽得率为7.22%。与模型组相比,多肽中、高剂量组均可显著提高小鼠血清、肝组织及脑组织中的T-SOD、GSH-Px活性(p<0.05),降低MDA的含量(p<0.05);其中高剂量组血清中T-SOD含量达到177.24 U/mL,MDA含量达到9.05 nmol/mL,GSH-Px达到261.59 U/mL,与空白对照组结果相近。研究结果表明,新疆伊吾县野山杏多肽对衰老模型小鼠具有良好的体内抗氧化作用。In this study,enzymatic hydrolysis was used to extract the peptides in wild apricot from Yiwu County of Xinjiang,and its antioxidant effect was examined in aging mouse model.Through single factor experiments,the effects of enzyme-substrate ratio,material-to-liquid ratio,extraction time,pH and enzymatic hydrolysis temperature on hydrolysis degree were examined,and the extraction process for the wild apricot peptide was optimized through combining single factor and orthogonal experiments.Taking the extract as the raw material,D-galactose was used to establish an aging mouse model,and the mice were divided into the blank control group,the low-,medium-,and high-dose wild apricot peptide groups(50,75 or 100 mg/kg),the aging model group and the Vc positive control group.After 30 days of continuous intragastric administration,the GSH-Px activity,SOD activity and MDA content in the serum,liver tissue and brain tissue homogenates were determined.The optimal conditions for extracting wild apricot peptides by enzymatic hydrolysis were as follows:enzyme-substrate ratio,4%;enzymolysis time,120 min;pH,8;enzymolysis temperature,65℃;material-liquid ratio,1:25.The hydrolysis degree and peptide yield were 35.73%and 7.22%,respectively.Compared with the model group,the medium-dose and high-dose peptide groups could significantly increase the activities of T-SOD and GSH-Px in mouse serum,liver tissue and brain tissue(p<0.05),and reduce the content of MDA(p<0.05).Among which,the serum contents of serum T-SOD,MDA and GSH-Px were 177.24 U/mL,9.05 nmol/mL and 261.59 U/mL.Respectively,in high dose groups,were similar to those of the blank control group.The results showed that the peptides from the wild apricot in Yiwu County of Xinjiang exhibited good anti-oxidant effects in the aging mouse model.

关 键 词：酶解法 生物活性肽 正交试验 抗氧化 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]