酶法制备透明质酸寡糖及其透皮吸收活性研究  

Preparation of Hyaluronic Acid Oligosaccharides by Enzymatic Method and Its Transdermal Absorption Activity

作　　者：李慧凝 张京良[2,3] 杨艮 江晓路 LI Huining;ZHANG Jingliang;YANG Gen;JIANG Xiaolu(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Marine Biomedical Research Institute of Qingdao,Qingdao 266007,China;Shandong Galaxy Bio-Tech Co.,Ltd.,Jining 273200,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]中国海洋大学医药学院,山东青岛266003 [3]青岛海洋生物医药研究院,山东青岛266007 [4]山东银河生物科技有限公司,山东济宁273200

出　　处：《食品工业科技》2022年第6期77-82,共6页Science and Technology of Food Industry

基　　金：山东省重点扶持区域人才项目“基于在线控制的酶法制备透明质酸寡糖关键技术研究与产业化”(370831SD200417313802)。

摘　　要：为研究透明质酸寡糖的透皮吸收活性,采用透明质酸裂解酶对透明质酸进行酶法降解,制备透明质酸寡糖,通过红外光谱与质谱对透明质酸寡糖进行分析,并对其吸湿性能、体表保湿性能和透皮吸收性能进行评价。结果显示,透明质酸裂解酶酶解制备透明质酸寡糖的最适底物浓度为1%,最适加酶量为3.0 U/mg,酶解制备透明质酸寡糖基本没有基团脱落,酶解产物以不饱和透明质酸二糖为主。酶法制备的透明质酸寡糖具有优良的体表保湿与透皮吸收性能,在生物化工领域具有广阔的应用前景。To investigate the transdermal absorption activity of hyaluronic acid oligosaccharides, hyaluronic acid oligosaccharides were prepared by enzymatic degradation of hyaluronic acid using hyaluronic acid lyase, and the hyaluronic acid oligosaccharides were analyzed by infrared spectroscopy and mass spectrometry, and their hygroscopic properties,body surface moisturizing properties and transdermal absorption properties were evaluated. The results showed that the optimum substrate concentration for the enzymatic preparation of hyaluronan oligosaccharides by hyaluronan lysis enzyme was 1%, and the optimum enzyme addition was 3.0 U/mg. The enzymatic preparation of hyaluronan oligosaccharides was free of moieties, and the enzymatic products were mainly unsaturated hyaluronan disaccharides. The enzymatically prepared hyaluronan oligosaccharide had excellent surface moisturizing and transdermal absorption properties, and would have a broad application prospect in the field of biochemicals.

关 键 词：透明质酸寡糖 透明质酸裂解酶 结构分析 透皮吸收活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]