枸杞黄酮提取方法的优化以及不同生态因子与枸杞黄酮相关性研究  

Optimization of Extraction Method of Lycium barbarum Flavonoids and the Correlation between Different Ecological Factors and Lycium barbarum Flavonoids

作　　者：王鹏波 谢晓蓉 代云云 刘建书 陈梅 张霞[2] 裴栋[2] 邸多隆 WANG Pengbo;XIE Xiaorong;DAI Yunyun;LIU Jianshu;CHEN Mei;ZHANG Xia;PEI Dong;DI Duolong(School of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences Key Laboratory of Northwestern Featured Plant Resources Chemistry,Chinese Academy of Sciences and Key Laboratory of Natural Medicines of Gansu Province,Lanzhou 730000,China;Functional Food Engineering Center of Shaanxi Province,Xi’an 710000,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000 [3]陕西功能食品工程中心有限公司,陕西西安710000

出　　处：《食品工业科技》2022年第6期236-242,共7页Science and Technology of Food Industry

基　　金：宁夏回族自治区重点研发计划重大重点项目(2019BEF02006);甘肃省重点研发计划项目(18YF1FA126);甘肃省科技厅重点研发计划项目(18YF1NA084)。

摘　　要：枸杞黄酮是中药材枸杞子主要的有效成分,也是其质量优劣的主要评价指标。本文分别考察了乙醇回流提取法、高速剪切辅助乙醇提取法、高速剪切辅助低共熔溶剂提取法、超声辅助低共熔溶剂提取法对枸杞黄酮提取量的影响,选出最佳的提取方法,通过单因素实验与正交试验获得最佳提取条件,采用SIMCA 14.1软件分析不同产区生态因子和枸杞黄酮含量的相关性。结果表明:高速剪切辅助低共熔溶剂提取枸杞黄酮的效率最优,最佳提取工艺为:转速14000 r/min,料液比1:20 g/mL,提取时间5 min,黄酮平均提取量为7.11 mg/g。不同产地枸杞黄酮含量从大到小依次为:宁夏银川>宁夏中卫>甘肃瓜州>宁夏固原>新疆精河>宁夏中宁>甘肃白银>甘肃武威>甘肃玉门>新疆阿尔泰>新疆伊宁>青海都兰>青海格尔木>韩国b>韩国a;由SIMCA 14.1软件分析得到:枸杞黄酮含量与温度和无霜期的正回归系数值最大,与海拔高度的负回归系数绝对值最大,影响枸杞黄酮含量最主要的生态因子是温度和无霜期。Lycium barbarum flavone is the main effective component of Chinese medicinal material Lycium barbarum, and it is also the main evaluation index of its quality. This paper investigated the effects of ethanol reflux extraction, high-speed shear-assisted ethanol extraction, high-speed shear-assisted DESs extraction, and ultrasound-assisted DESs extraction on the extraction amount of Lycium barbarum flavonoids. The best extraction method was selected, and the best extraction conditions were obtained by single factor experiments and orthogonal experiment. Based on the best extraction method,SIMCA 14.1 was used. The software analyzed the correlation between the ecological factors in different producing areas and the content of Lycium barbarum flavonoids. The results showed that the extraction efficiency of Lycium barbarum flavonoids with high-speed shearing assisted DESs was the best, the optimal extraction process was as follows: Speed14000 r/min, material-to-liquid ratio 1:20 g/mL, extraction time 5 min, the average flavonoid extraction volume was7.11 mg/g. The flavonoid content of Lycium barbarum from different origins in descending order was: Ningxia Yinchuan>Ningxia Zhongwei>Gansu Guazhou>Ningxia Guyuan>Xinjiang Jinghe>Ningxia Zhongning>Gansu Baiyin>Gansu Wuwei>Gansu Yumen>Xinjiang Altai>Xinjiang Yining>Qinghai Dulan>Qinghai Golmud>South Korea b>South Korea a. SIMCA 14.1 Software analysis showed that the positive regression coefficient value of Lycium barbarum flavonoids content and temperature and frost-free period was the largest, and the absolute value of negative regression coefficient with altitude was the largest. The most important ecological factors affecting the content of Lycium barbarum flavonoids were temperature and frost-free period.

关 键 词：枸杞黄酮提取 低共熔溶剂 高速剪切 温度 海拔 生态因子 

分 类 号：TS201.2[轻工技术与工程—食品科学]