芦柑叶多酚超声波辅助提取工艺优化及其对亚硝酸盐的清除活性研究  

Ultrasonic-assisted Extraction of Polyphenols from Ponkan Leaves and Its Nitrite Scavenging Activity

作　　者：苏火煌[1,2] SU Huo-huang(College of Applied Chemical Engineering,Zhangzhou Institute of Technology,Zhangzhou 363000,China;Collaborative Innovation Center of Fine Chemicals in Fujian Province,Zhangzhou 363000,China)

机构地区：[1]漳州职业技术学院应用化工学院,福建漳州363000 [2]福建省精细化工应用技术协同创新中心,福建漳州363000

出　　处：《长春师范大学学报》2022年第2期75-83,共9页Journal of Changchun Normal University

基　　金：福建省中青年教师教育科研项目“漳州芦柑叶总黄酮的提取及活性研究”(JAT191412);漳州职业技术学院科研计划资助项目“漳州紫玉兰叶有效成分的提取及活性研究”(ZZY2021B121)。

摘　　要：对芦柑叶多酚的超声波辅助提取工艺进行响应面法优化,并研究芦柑叶多酚对亚硝酸盐的清除活性。先考察了乙醇浓度、超声时间、液料比和超声温度四个单因素对芦柑叶多酚提取率的影响,再结合响应面法对提取工艺条件进行优化,最后考察了芦柑叶多酚对亚硝酸盐的清除能力。结果表明,芦柑叶多酚的最佳提取工艺为:乙醇浓度61%,超声时间50 min,液料比20 mL/g和超声温度63℃,测得芦柑叶多酚的提取率为32.27 mg/g,其与模型预测的理论值(32.58 mg/g)相对误差为0.95%,说明所建立的模型方程准确、可靠,可应用于芦柑叶多酚提取工艺的预测。在所考察的质量浓度范围内,芦柑叶多酚均能较好地清除亚硝酸盐。多酚的质量浓度与亚硝酸盐的清除率成正比,芦柑叶多酚对亚硝酸盐清除的IC_(50)为1.93 mg/L,为芦柑叶资源开发成一种新型的天然亚硝酸盐清除剂提供了科学依据。The ultrasonic-assisted extraction process of polyphenols from Ponkan leaves was optimized by response surface methodology,and the scavenging activity of polyphenols on nitrite was studied.Firstly,the effects of four single factors of ethanol concentration,ultrasonic time,liquid-to-material ratio and ultrasonic temperature on the extraction yield of polyphenols from Ponkan leaves were investigated.Then,the extraction process conditions were optimized with response surface methodology.Finally,the ability of Ponkan leaves polyphenols to remove nitrite was investigated.The results showed that the optimal extraction process for the polyphenols from Ponkan leaves was as follows:ethanol concentration was 61%,ultrasonic time was 50 min,liquid-to-material ratio was 20 mL/g,and ultrasonic temperature was 63℃.The extraction yield of polyphenols from Ponkan leaves was 32.27 mg/g,its relative error with the theoretical value 32.58 mg/g predicted by the model was 0.95%,indicating that the established model equation was accurate and reliable,and could be applied to the prediction of the extraction process of Ponkan leaves polyphenols.Within the concentration range investigated,the polyphenols in the Ponkan leaves could remove nitrite well,and the concentration of polyphenols was directly proportional to the removal rate of nitrite.The IC_(50) of Ponkan leaves polyphenols for nitrite removal was 1.93 mg/L,which provided a scientific basis for the development of Ponkan leaves resources into a new type of natural nitrite scavenger.

关 键 词：芦柑叶 多酚 提取 亚硝酸盐 清除活性 

分 类 号：TS202[轻工技术与工程—食品科学] TQ91[轻工技术与工程—食品科学与工程]