自制核心耗材促进大型仪器设备在实验教学中的应用  

Promotion of Application of Large-scale Instruments in Experimental Teaching by Self-made Core Consumables

作　　者：万建 汪兵 周权[1,2,3] 阮君[1,2,3] 张迪 李兵 WAN Jian;WANG Bing;ZHOU Quan;RUAN Jun;ZHANG Di;LI Bing(College of life Science,Central China Normal University,Wuhan 430079,China;Modal Experiment Teaching Center of Hubei for Province Biology,Central China Normal University,Wuhan 430079,China;National Biology Virtual Simulation Experiment Teaching Center,Central China Normal University,Wuhan 430079,China)

机构地区：[1]华中师范大学生命科学学院,武汉430079 [2]华中师范大学湖北省生物学实验教学示范中心,武汉430079 [3]华中师范大学国家级生物学虚拟仿真实验教学示范中心,武汉430079

出　　处：《实验室研究与探索》2022年第1期162-164,253,共4页Research and Exploration In Laboratory

基　　金：湖北省高等学校实验室研究项目(HBSY2020-18);华中师范大学校级研究项目(202016,202077)。

摘　　要：通过技术攻关自制核心耗材、实验项目构建贴近课程需求、创新课堂以赛促学等方式建立了基于高分辨液相色谱质谱联用技术的蛋白质磷酸化修饰鉴定教学实验。将生物学前沿技术手段与方法应用于实验教学中,使学生了解当前生命科学领域研究热点、研究思路与实验方法,拓宽学生专业视野,提高生物专业学生对科研的积极性。The teaching experiment of protein phosphorylation modification identification based on high resolution liquid chromatograph-mass spectrometry(HPLC-MS)was established by making self-made core consumables through technical breakthroughs,constructing experimental items close to the curriculum needs,and promoting learning through innovative classroom methods.The application of cutting-edge biological technology and methods in experimental teaching enables students to understand the current research hotspots,research ideas and methods in the field of life science,broaden students’professional vision,and improve the enthusiasm of biology students for scientific research.

关 键 词：高分辨质谱 实验教学 大型仪器 磷酸化修饰 

分 类 号：G641[文化科学—高等教育学] Q7[文化科学—教育学]