紫茎泽兰入侵前后根部蜡样芽孢杆菌含量比较及其对化感物质处理的响应  

Abundance of Bacillus cereus in Ageratina adenophora roots before and after its invasion and response to allelochemicals

作　　者：赵云鹏 杨子豪 徐刚 Esteban Rodríguez-Leyva 杨国庆[1] ZHAO Yunpeng;YANG Zihao;XU Gang;Esteban Rodríguez-Leyva;YANG Guoqing(Horticulture and Plant Protection College,Yangzhou University,Yangzhou,Jiangsu 225009,China;Posgrado en Fitosanidad,Entomología y Acarología,Colegio de Postgraduados,Texcoco,Mexico)

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]Posgrado en Fitosanidad,Entomología y Acarología,Colegio de Postgraduados,Texcoco,Mexico

出　　处：《生物安全学报》2022年第1期27-33,共7页Journal of biosafety

基　　金：国家自然科学基金(31772229)。

摘　　要：【目的】比较紫茎泽兰入侵地种群根部有益菌蜡样芽孢杆菌含量与原产地种群的差异,及其对化感物质的响应,为揭示紫茎泽兰与有益菌互作在入侵前后的变化及其机制提供参考。【方法】分别以紫茎泽兰原产地墨西哥的3个种群(M1、M2、M3)和入侵地云南的3个种群(C1、C2、C3)为材料,采用同质园试验方法和蜡样芽孢杆菌分子定量的技术,检测原产地和入侵地紫茎泽兰种群植株根部(根系、根际土壤)蜡样芽孢杆菌的含量;使用不同种群紫茎泽兰植株叶片淋溶液和根系分泌液处理入侵地土壤,检测化感物质对土壤中蜡样芽孢杆菌含量的影响。【结果】紫茎泽兰入侵地种群根系蜡样芽孢杆菌含量高于原产地种群,其中入侵地种群C2的含量比原产地种群M1高1172%;在根际土壤中,2年生紫茎泽兰入侵地3个种群的蜡样芽孢杆菌含量显著高于原产地种群M1和M3。紫茎泽兰叶片淋溶液和根系分泌液处理均促进了土壤蜡样芽孢杆菌含量增加,入侵地种群叶片淋溶液处理的土壤的蜡样芽孢杆菌增加量高于原产地种群,根系分泌液处理的后期测定中也如此。【结论】与原产地相比,紫茎泽兰入侵后,根系与根际土壤蜡样芽孢杆菌含量均有所增加,增强了有益菌对入侵地种群的正反馈效应。【Aim】 The abundance of the beneficial bacterium—Bacillus cereus in roots(in-root, rhizosphere soil) of Ageratina adenophora(invasive and native populations) and its response to plant allelochemicals were determined. The findings of this study aimed to provide a scientific basis for revealing the changes and mechanisms of the interactions between the invasive A. adenophora and beneficial bacteria. 【Method】 Three native and three invasive populations of A. adenophora plants from Mexico(M, invasive) and Yunnan(C, native) were used in this study. The common garden method and molecular quantification technology were used to detect the abundance of B. cereus in the roots of native and invasive plants. The effects of allelochemicals on the abundance of B. cereus in soil were investigated using leaf leachates and root exudates of A. adenophora plants from all six populations. 【Result】 The abundance of B. cereus in the roots of the invasive populations of A. adenophora were higher than those of the native populations, and the abundance from the invasive population C2 was 1172% higher than that of the native population M1. In the rhizosphere soil, the abundance of B. cereus in the three invasive populations was higher than that in the two native populations(M1 and M3), two years from planting. Treatment with leaf leachates and root exudates from different populations of A. adenophora increased the abundance of B. cereus in soil. 【Conclusion】 The abundance of B. cereus in the roots and rhizosphere soil increased when invasive A. adenophora is grown, which could enhance the positive feedback effects of beneficial bacteria on invasive plants.

关 键 词：紫茎泽兰 蜡样芽孢杆菌 化感物质 入侵 

分 类 号：S451[农业科学—植物保护]