超高效合相色谱(UPC^(2))技术快速测定植物油中的维生素A和维生素E  

Rapid determination of vitamin A and vitamin E in vegetable oil based on ultra high performance convergence chromatography(UPC^(2))

作　　者：彭嬿雯 王波 刘阿静 王莹捷 把灵珍 韩舜愈[1] 管贤贤 周小平 PENG Yanwen;WANG Bo;LIU Ajing;WANG Yingjie;BA Lingzhen;HAN Shunyu;GUAN Xianxian;ZHOU Xiaoping(School of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Lanzhou Customs Technology Center,Lanzhou 730000,China;College of Geography and Environmental Science,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,兰州730070 [2]兰州海关技术中心,兰州730000 [3]西北师范大学地理与环境科学学院,兰州730070

出　　处：《中国油脂》2022年第1期147-152,共6页China Oils and Fats

摘　　要：为了使植物油中脂溶性维生素E异构体得到更好的分离,建立一种超高效合相色谱(UPC^(2))快速分离和测定植物油中维生素A和维生素E的方法。样品经异丙醇溶解,过0.22μm有机相滤膜后直接进样分析。分析条件为HSS C_(18)SB色谱柱(3.0 mm×100 mm, 1.8μm),CO_(18)和0.2%甲酸甲醇溶液为流动相,梯度洗脱,流速1.0 mL/min,柱温50℃,检测波长290 nm。结果表明:维生素A和维生素E在测定范围内呈现良好的线性关系,相关系数均在0.999 5及以上,样品回收率为82.00%~106.82%,RSD在0.94%~3.57%之间,日内精密度与日间精密度的RSD分别为1.94%~3.51%与3.74%~5.13%,并采用已建立的方法对26份植物油样品中的维生素A和维生素E含量进行测定,表明植物油中维生素E含量最高,其中以α-生育酚和γ-生育酚为主,β-生育酚含量较低。该方法简单高效、灵敏度高、重复性好,可用于植物油中脂溶性维生素A和维生素E的分离检测。In order to better separate the fat-soluble vitamin E isomers in vegetable oils, a method for rapid separation and determination of vitamin A and vitamin E in vegetable oil by ultra high performance convergence chromatography was established. The sample was dissolved in isopropanol and passed through a 0.22 μm organic phase filter membrane, and then directly injected for analysis. The analysis conditions were as follows: HSS C_(18)SB chromatographic column(3.0 mm×100 mm, 1.8 μm), with CO_(2) and 0.2% formic acid methanol solution as mobile phase, gradient elution, flow rate 1.0 mL/min, column temperature 50℃, detection wavelength 290 nm. The results showed that vitamin A and vitamin E showed a good linear relationships in the determination range, the correlation coefficients were not less than 0.999 5, the sample recovery was 82.00%-106.82%, the RSD was 0.94%-3.57%, and the RSD of intraday precision and daytime precision were 1.94%-3.51% and 3.74%-5.13%, respectively. The contents of vitamin A and vitamin E in 26 vegetable oil samples were determined by the established method. The vitamin E content was the highest, in which α-tocopherol and γ-tocopherol was the main tocopherol, while β-tocopherol conternt was lower. The method is simple, efficient, sensitive and reproducible. The method can be used for the separation and determination of vitamin A and vitamin E in vegetable oil.

关 键 词：超高效合相色谱法 植物油 维生素A 维生素E 

分 类 号：O657.72[理学—分析化学] TS225.1[理学—化学]